Zeta Potential Measurement
Zeta Potential Measurement
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DOI：
10.1007/978-1-60327-198-1_6
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Patri, Anil K.
中科院分区：
文献类型：
--
作者：
Clogston, Jeffrey D.;Patri, Anil K.
本章介绍了一种测量溶液中纳米颗粒周围双电层静电势的方法。这被称为zeta电位。ζ电位在-10和+10 mV之间的纳米颗粒被认为是近似中性的，而ζ电位大于+30 mV或小于-30 mV的纳米颗粒分别被认为是强阳离子和强阴离子。由于大多数细胞膜是带负电荷的，ζ电位可以影响纳米颗粒渗透膜的趋势，阳离子颗粒通常显示出与细胞壁破坏相关的更多毒性。该技术在生物应用中常用的两种类型的纳米颗粒中得到了证明：胶体金（强阴离子）和胺封端的PAMAM树枝状聚合物（强阳离子）。
This chapter describes a method for the measurement of the electrostatic potential at the electrical double layer surrounding a nanoparticle in solution. This is referred to as the zeta potential. Nanoparticles with a zeta potential between -10 and +10 mV are considered approximately neutral, while nanoparticles with zeta potentials of greater than +30 mV or less than -30 mV are considered strongly cationic and strongly anionic, respectively. Since most cellular membranes are negatively charged, zeta potential can affect a nanoparticle's tendency to permeate membranes, with cationic particles generally displaying more toxicity associated with cell wall disruption. This technique is demonstrated for two types of nanoparticles commonly used in biological applications: colloidal gold (strongly anionic) and amine-terminated PAMAM dendrimer (strongly cationic).