Control of plant growth and development by overexpressing MAP3K17, an ABA-inducible MAP3K, in Arabidopsis
Control of plant growth and development by overexpressing MAP3K17, an ABA-inducible MAP3K, in Arabidopsis
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DOI：
10.5511/plantbiotechnology.18.0412a
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发表时间：
2018-06-01
影响因子：
1.6
通讯作者：
Nanmori, Takashi
中科院分区：
文献类型：
--
作者：
Matsuoka, Daisuke;Soga, Kaori;Nanmori, Takashi
脱落酸（阿坝）在植物生长发育和逆境响应中起着重要作用。阿坝调节植物生长和发育的许多方面，包括种子成熟、休眠、萌发、从营养生长到生殖生长的转变、叶片衰老以及对环境胁迫（如干旱、高盐和寒冷）的响应。还已知丝裂原活化蛋白激酶（MAPK）级联在阿坝信号传导中起作用。最近，我们和另一个小组已经鉴定了ABA诱导的MAP 3 Ks MAP 3 K17和MAP 3 K18作为MKK 3的上游MAP 3 Ks，暗示MAP 3 K17/18-MKK 3-MPK 1/2/7/14级联在阿坝信号传导中。还有报道称，拟南芥中MAP 3 K18的过表达会导致叶片提前衰老表型、阿坝超敏气孔关闭和耐旱性。在这项研究中，我们产生了过表达MAP 3 K17（35 S：MAP 3 K17）及其激酶失活形式（35 S：MAP 3 K17 KN）的转基因植物。35 S：MAP 3 K17的抽薹早于WT，且幼苗鲜重较小，而35 S：MAP 3 K17 KN则表现出相反的表型。这些结果表明，从营养生长到生殖生长的过渡可以通过过表达的MAP 3 K17及其激酶失活形式进行调节。而且，35 S：MAP 3 K17在萌发后的生长过程中表现出对阿坝较低的敏感性，而35 S：MAP 3 K17 KN表现出相反的表型，表明MAP 3 K17在阿坝响应中起负作用。我们的工作提供了通过基因操作ABA诱导的MAPK级联来调节植物生长和发育的可能性，从而改善作物生长和生产力。
Abscisic acid (ABA) plays an important role in plant growth, development, and stress responses. ABA regulates many aspects of plant growth and development, including seed maturation, dormancy, germination, the transition from vegetative to reproductive growth, leaf senescence and responses to environmental stresses, such as drought, high salinity and cold. It is also known that mitogen-activated protein kinase (MAPK) cascades function in ABA signaling. Recently, we and another group have identified the ABA-inducible MAP3Ks MAP3K17 and MAP3K18 as the upstream MAP3Ks of MKK3, implicating the MAP3K17/18-MKK3-MPK1/2/7/14 cascade in ABA signaling. It has also been reported that overexpression of MAP3K18 in Arabidopsis causes an early leaf senescence phenotype, ABA hypersensitive stomata closing, and drought tolerance. In this study, we generated transgenic plants overexpressing MAP3K17 (35S:MAP3K17) and its kinase-inactive form (35S:MAP3K17KN). The bolting of 35S:MAP3K17 was earlier than WT, and the fresh weights of the seedlings were smaller, whereas 35S: MAP3K17KN showed the opposite phenotype. These results indicate that the transition from vegetative to reproductive growth can be regulated by overexpression of MAP3K17 and its kinase-inactive form. Moreover, 35S: MAP3K17 showed lower sensitivity to ABA during post-germinated growth, whereas 35S:MAP3K17 KN showed the opposite phenotype, suggesting the negative roles of MAP3K17 in the response to ABA. Our work provides the possibility to regulate plant growth and development by the genetic manipulation of ABA-induced MAPK cascades, leading to improved crop growth and productivity.