Interleukin-22 ameliorates liver fibrosis through miR-200a/beta-catenin.
Interleukin-22 ameliorates liver fibrosis through miR-200a/beta-catenin.
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Interleukin-22 通过 miR-200a/β-catenin 改善肝纤维化
DOI：
10.1038/srep36436
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发表时间：
2016-11-07
影响因子：
4.6
通讯作者：
Jiang HX
中科院分区：
文献类型：
--
作者：
Hu BL;Shi C;Lei RE;Lu DH;Luo W;Qin SY;Zhou Y;Jiang HX
IL-22通过抑制肝星状细胞（HSC）来改善肝纤维化，并且miR-200 a的缺失与肝纤维化的发展相关。本研究旨在探讨IL-22和miR-200 a在体内和体外调节肝纤维化中的相互作用。我们观察到IL-22显著降低HSC的增殖并增加p-STAT 3的表达。荧光素酶报告基因检测证实β-catenin是miR-200 a的靶基因，上调miR-200 a可显著抑制HSC增殖，降低β-catenin的表达。IL-22处理增加HSC中miR-200 a的表达并降低HSC中β-catenin的表达。IL-22处理后，肝纤维化大鼠肝脏中p-STAT 3和miR-200 a的表达升高，而β-catenin的表达降低。β-catenin和p-STAT 3在肝纤维化大鼠肝脏和HSC中的表达水平呈负相关。β-catenin上调可抑制HSC中p-STAT 3的表达。结论IL-22可能通过上调miR-200 a的表达，下调β-catenin的表达，抑制HSC活化，减轻肝纤维化，提示IL-22/STAT 3和β-catenin通路之间可能存在交叉作用。
IL-22 ameliorates liver fibrosis by inhibiting hepatic stellate cells (HSC), and loss of miR-200a is associated with the development of liver fibrosis. The study aimed to investigate the interplay between IL-22 and miR-200a in regulating liver fibrosisin vivoandin vitro. We observed that IL-22 significantly reduced the proliferation of HSC and increased the expression of p-STAT3. β-catenin was identified as a target gene of miR-200a by luciferase reporter assay, and upregulation of miR-200a significantly attenuated the proliferation of HSC and reduced β-catenin expression. IL-22 treatment increased expression of miR-200a and decreased expression of β-catenin in HSC. The expression of p-STAT3 and miR-200a was elevated while β-catenin was decreased in fibrotic rat liver after IL-22 treatment. Expression levels of β-catenin and p-STAT3 were inversely correlated in fibrotic rat liver and HSC. Upregulation of β-catenin suppressed expression of p-STAT3 in HSC. We concluded that IL-22 inhibits HSC activation and ameliorates liver fibrosis through enhancing expression of miR-200a and reducing expression of β-catenin, suggesting there may be a crosstalk between IL-22/STAT3 and β-catenin pathway.
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影响因子：
3.3
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
Gao, Bin
影响因子：
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作者：
Hassan, Zeinab Korany;Al-Olayan, Ebtisam M.
通讯作者：
Al-Olayan, Ebtisam M.