Antibacterial activity of bacillomycin D-like compounds isolated from Bacillus amyloliquefaciens HAB-2 against Burkholderia pseudomallei
Antibacterial activity of bacillomycin D-like compounds isolated from Bacillus amyloliquefaciens HAB-2 against Burkholderia pseudomallei
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DOI：
10.4103/2221-1691.280295
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发表时间：
2020-04
影响因子：
1.7
通讯作者：
M. Rajaofera;Xun Kang;Pengfei Jin;Xin Chen;Chenchu Li;Lijun Yin;Lin Liu;Qing-hui Sun;N. Zhang;Chuizhe Chen;Na He;Qianfeng Xia;W. Miao
中科院分区：
文献类型：
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作者：
M. Rajaofera;Xun Kang;Pengfei Jin;Xin Chen;Chenchu Li;Lijun Yin;Lin Liu;Qing-hui Sun;N. Zhang;Chuizhe Chen;Na He;Qianfeng Xia;W. Miao
目的：研究中药复方对类鼻疽伯克霍尔德菌（B. pseudomallei）菌株HNBP 001，其为从解淀粉芽孢杆菌HAB-2分离的名为芽孢杆菌霉素DC的芽孢杆菌霉素D样环状脂肽化合物。方法：采用B.使用纸片扩散法测定假鼻疽。采用微量稀释法测定最低抑菌浓度。此外，进行透射电子显微镜和定量实时聚合酶链反应测定，以确定MexB，OprD 2和qnrS基因的表达。结果：芽孢杆菌素DC对B有明显的抑菌圈。对类鼻疽的最低抑菌浓度在处理后24 h为12.5 μg/mL，在48和72 h为50 μg/mL。透射电镜观察发现，芽孢杆菌素DC可使细胞表面粗糙，细胞膜损伤。定量实时聚合酶链反应分析显示MexB、OprD 2和qnrS基因的低表达。结论：芽孢杆菌素DC对B细胞生长有抑制作用。可作为B类抗生素的新候选药物。假鼻疽
Objective: To investigate the inhibitory effect on Burkholderia pseudomallei (B. pseudomallei) strain HNBP001 of a bacillomycin D-like cyclic lipopeptide compound named bacillomycin DC isolated from Bacillus amyloliquefaciens HAB-2. Methods: The antibacterial effect of bacillomycin DC on B. pseudomallei was determined using the disk diffusion method. The minimum inhibitory concentrations were evaluated by microdilution assay. In addition, transmission electron microscopy was performed and quantitative real-time polymerase chain reaction assay was carried out to determine the expression of MexB, OprD2, and qnrS genes. Results: Bacillomycin DC produced an inhibition zone against B. pseudomallei with minimum inhibitory concentration values of 12.5 μg/mL 24 h after treatment and 50 μg/mL at 48 and 72 h. Transmission electron microscopy showed that bacillomycin DC resulted in roughening cell surface and cell membrane damage. Quantitative real-time polymerase chain reaction analysis showed low expression of MexB, OprD2 and qnrS genes. Conclusions: Bacillomycin DC inhibits the growth of B. pseudomallei and can be a new candidate for antimicrobial agents of B. pseudomallei.