Virus-specific CD8+ T cells in primary and secondary influenza pneumonia
Virus-specific CD8+ T cells in primary and secondary influenza pneumonia
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DOI：
10.1016/s1074-7613(00)80573-7
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发表时间：
1998-06-01
期刊：
影响因子：
32.4
通讯作者：
Doherty, PC
中科院分区：
文献类型：
--
作者：
Flynn, KJ;Belz, GT;Doherty, PC
病毒特异性CD8(+)效应T细胞(ECTL)在原发性流感肺炎小鼠的肺中丰富，尽管后来用基于多肽的染色方法在纵隔淋巴结(MLN)或脾中检测到记忆T细胞(MCTL)是在流式细胞术分析的限制。在H1N1疫苗接种几个月后，用H3N2病毒进行呼吸道攻击会引起大规模的召回反应，这会比NAVE对照组提前2-3天降低病毒滴度。流感特异性mCTL在6小时内产生干扰素-γ，但仍需要4-5天才能定位到受感染的呼吸道。这一延迟反映了召回反应首先在MLN中发展，而MLN中包含的mctl相对较少。在二次攻击后，对次要表位的反应不那么明显。
Virus-specific CD8(+) effector T cells (eCTL) are enriched in the lungs of mice with primary influenza pneumonia, though later detection of memory T cells (mCTL) in the mediastinal lymph nodes (MLN) or spleen by peptide-based staining protocols is at the limits of flow cytometric analysis. Respiratory challenge with an H3N2 virus months after H1N1 priming induces a massive recall response, which reduces virus titers 2-3 days earlier than in nave controls. Influenza-specific mCTL produce interferon-gamma within 6 hr, but still take 4-5 days to localize to the infected respiratory tract. The delay reflects that the recall response develops first in the MLN, which contains relatively few mCTL. The response to a subdominant epitope is less obvious after secondary challenge.