Cloning and characterization of a glutathione S-transferase induced by a herbicide safener in barley (Hordeum vulgare)
Cloning and characterization of a glutathione S-transferase induced by a herbicide safener in barley (Hordeum vulgare)
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DOI：
10.1034/j.1399-3054.2002.1160308.x
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发表时间：
2002-11-01
影响因子：
6.4
通讯作者：
Roulet, A
中科院分区：
文献类型：
--
作者：
Scalla, R;Roulet, A
研究了除草剂甲氰菊酯对黑大麦谷胱甘肽S转移酶(GST，EC 2.5.1.18)活性的影响。Alexis)进行了检查。以1-氯-2，4-二硝基苯(CDNB)和除草剂苯恶草为底物，甲氰菊酯-二乙基处理提高了GST活性。谷胱甘肽(GSH)过氧化物酶活性明显升高。GST亚基的分析采用高效液相色谱仪。更安全的处理增加了两个主要亚基的数量，而另外两个主要亚基的数量保持不变。克隆了富含诱导型谷胱甘肽S转移酶(HvGST6)的基因，并在大肠杆菌中进行了表达。纯化后的酶以除草剂为底物，活性较低。相反，它表现出很强的GSH过氧化物酶活性。
The effect of the herbicide safener mefenpyr-diethyl on glutathione S -transferase (GST, EC 2.5.1.18) activities of dark-grown barley (Hordeum vulgare cv. Alexis) was examined. Mefenpyr-diethyl treatment increased the GST activity with 1-chloro-2,4-dinitrobenzene (CDNB) and the herbicide fenoxaprop as substrates. Glutathione (GSH) peroxidase activity was markedly increased. GST subunits were analysed by high performance liquid chromatography (HPLC). The quantities of two major subunits were increased by the safener treatment, while the quantities of two other major subunits remained constant. A cDNA encoding the most abundant inducible GST (HvGST6) was cloned and expressed in E. coli . The purified enzyme exhibited a low activity with herbicides as substrates. By contrast, it exhibited a strong GSH peroxidase activity.