Rapid Amygdala Kindling Causes Motor Seizure and Comorbidity of Anxiety- and Depression-Like Behaviors in Rats.
Rapid Amygdala Kindling Causes Motor Seizure and Comorbidity of Anxiety- and Depression-Like Behaviors in Rats.
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杏仁核快速点燃会导致大鼠运动发作以及焦虑和抑郁样行为的共病。
DOI：
10.3389/fnbeh.2016.00129
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发表时间：
2016
影响因子：
3
通讯作者：
Shaw FZ
中科院分区：
文献类型：
--
作者：
Chen SD;Wang YL;Liang SF;Shaw FZ
杏仁核点燃是伴有抽搐的颞叶癫痫（TLE）模型。杏仁核快速点燃对于运动性癫痫发作的快速发展和抗癫痫药物筛选具有优势。杏仁核的快速点燃会导致癫痫发生，并伴有幼鼠早期隔离时的抗焦虑反应或未成熟大鼠的抑郁行为。然而，在正常繁殖的成年大鼠中，杏仁核快速点燃对焦虑和抑郁样行为共病的影响尚未被探索。在本研究中，在 2 天内对成年 Wistar 大鼠进行 40 次杏仁核刺激。记录整个杏仁核点火过程中的放电后（AD）和癫痫发作阶段。通过高架十字迷宫（EPM）测试和旷场（OF）测试评估焦虑样行为，而通过强迫游泳（FS）和蔗糖消耗（SC）测试评估抑郁样行为。 kindle组引发了强直阵挛性惊厥。快速杏仁核点燃导致进入EPM张开臂或OF中心区域的开放区域的频率显着降低，蔗糖摄入量较低，并且在kindle组中FS测试的不动时间更长。我们的结果表明，杏仁核快速点燃会引发严重的运动性癫痫发作，并伴有焦虑和抑郁样行为。
Amygdala kindling is a model of temporal lobe epilepsy (TLE) with convulsion. The rapid amygdala kindling has an advantage on quick development of motor seizures and for antiepileptic drugs screening. The rapid amygdala kindling causes epileptogenesis accompanied by an anxiolytic response in early isolation of rat pups or depressive behavior in immature rats. However, the effect of rapid amygdala kindling on comorbidity of anxiety- and depression-like behaviors is unexplored in adult rats with normal breeding. In the present study, 40 amygdala stimulations given within 2 days were applied in adult Wistar rats. Afterdischarge (AD) and seizure stage were recorded throughout the amygdala kindling. Anxiety-like behaviors were evaluated by the elevated plus maze (EPM) test and open field (OF) test, whereas depression-like behaviors were assessed by the forced swim (FS) and sucrose consumption (SC) tests. A tonic-clonic convulsion was provoked in the kindle group. Rapid amygdala kindling resulted in a significantly lower frequency entering an open area of either open arms of the EPM or the central zone of an OF, lower sucrose intake, and longer immobility of the FS test in the kindle group. Our results suggest that rapid amygdala kindling elicited severe motor seizures comorbid with anxiety- and depression-like behaviors.