Up-regulation of acyl-coenzyme A:cholesterol acyltransferase-1 by transforming growth factor-β1 during differentiation of human monocytes into macrophages
Up-regulation of acyl-coenzyme A:cholesterol acyltransferase-1 by transforming growth factor-β1 during differentiation of human monocytes into macrophages
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DOI：
10.1016/j.bbrc.2004.05.190
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发表时间：
2004-07-23
影响因子：
3.1
通讯作者：
Horiuchi, S
中科院分区：
文献类型：
--
作者：
Hori, M;Miyazaki, A;Horiuchi, S
在人单核细胞向巨噬细胞分化过程中酰基辅酶A：胆固醇酰基转移酶-1（ACAT-1）的表达增加。为了进一步阐明巨噬细胞中ACAT-1的调节机制，我们检测了包括转化生长因子-β 1（TGF-β 1）在内的五种细胞因子对培养的人单核-巨噬细胞中ACAT-1表达的影响。免疫印迹分析表明，在人单核细胞分化为巨噬细胞的过程中加入TGF-β 1可使ACAT-1蛋白表达增加2 - 3倍。ACAT活性平行增加1.8倍。北方印迹分析显示，在检测到的三种ACAT-1 mRNA转录本（2.8-、3.6-和4.3-kb）中，TGF-β 1选择性增加2.8-和3.6-kb转录本。当分化后加入TGF-β 1时，ACAT-1的表达没有改变。由于TGF-β 1在人类动脉粥样硬化病变中表达，目前的结果表明，ACAT-1在从循环浸润到血管壁的单核细胞中的表达可能被预先存在的TGF-β 1增强。(C)2004年爱思唯尔公司All rights reserved.
Expression of acyl-coenzyme A:cholesterol acyltransferase-1 (ACAT-1) increases during differentiation of human monocytes into macrophages. To further elucidate the mechanism for ACAT-1 regulation in macrophages, we examined the effects of five cytokines including transforming growth factor-beta1 (TGF-beta1) on ACAT-1 expression in cultured human monocyte-macrophages. Immunoblot analyses showed that TGF-beta1 increased ACAT-1 protein expression by two- to threefold when added during differentiation of human monocytes into macrophages. ACAT activity increased in parallel by 1.8-fold. Northern blot analyses revealed that among the three ACAT-1 mRNA transcripts detected (2.8-, 3.6-, and 4.3-kb), the 2.8- and 3.6-kb transcripts were selectively increased by TGF-beta1. When TGF-beta1 was added after differentiation, ACAT-1 expression was not altered. Since TGF-beta1 is expressed in human atherosclerotic lesions, the current results suggest that ACAT-1 expression in monocytes infiltrating from the circulation to vascular walls may be enhanced by pre-existing TGF-beta1. (C) 2004 Elsevier Inc. All rights reserved.