Highly Efficient and Ultra-small Volume Separation by Pressure-Driven Liquid Chromatography in Extended Nanochannels
Highly Efficient and Ultra-small Volume Separation by Pressure-Driven Liquid Chromatography in Extended Nanochannels
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DOI：
10.1002/smll.201102420
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发表时间：
2012-04-23
期刊：
影响因子：
13.3
通讯作者：
Kitamori, Takehiko
中科院分区：
文献类型：
--
作者：
Ishibashi, Ryo;Mawatari, Kazuma;Kitamori, Takehiko
在纳米流体分析，这是用来检查液体的量从阿升到毫微微升规模的快速发展的兴趣，与最近的兴趣减少样品量，如在单细胞分析领域。对于一般的纳米流体分析，压力驱动流不限制溶剂（水性或有机）的选择的事实是重要的。这项研究显示了第一个压力驱动的液相色谱技术，能够分离阿托至毫微微升的样品体积，在几秒钟内具有高分离效率。的表观扩散系数的测量的reretentive样品表明，有甲苯的粘度在扩展的纳米空间中没有增加，不像在水性溶剂。因此，正常相分离的评估应仅涉及检查扩展纳米空间的小尺寸的影响。与常规填充的高效液相色谱柱相比，这里的分离导致分离速度（4s）快2个数量级，进样体积（100 fL）小9个数量级，分离效率（440 000板/m）高1个数量级。此外，分离行为与理论一致，表明这种高效率是由于分离通道的小且受控的尺寸，其中通过通道深度方向的扩散足够快而可以忽略。我们基于芯片的平台应该允许直接和实时分析或筛选超低体积的样品。
The rapidly developing interest in nanofluidic analysis, which is used to examine liquids ranging in amounts from the attoliter to the femtoliter scale, correlates with the recent interest in decreased sample amounts, such as in the field of single-cell analysis. For general nanofluidic analysis, the fact that a pressure-driven flow does not limit the choice of solvents (aqueous or organic) is important. This study shows the first pressure-driven liquid chromatography technique that enables separation of atto- to femtoliter sample volumes, with a high separation efficiency within a few seconds. The apparent diffusion coefficient measurement of the unretentive sample suggests that there is no increase in the viscosity of toluene in the extended nanospace, unlike in aqueous solvents. Evaluation of the normal phase separation, therefore, should involve only the examination of the effect of the small size of the extended nanospace. Compared to a conventionally packed high-performance liquid chromatography column, the separation here results in a faster separation (4 s) by 2 orders of magnitude, a smaller injection volume (100 fL) by 9 orders, and a higher separation efficiency (440 000 plates/m) by 1 order. Moreover, the separation behavior agrees with the theory showing that this high efficiency was due to the small and controlled size of the separation channel, where the diffusion through the channel depth direction is fast enough to be neglected. Our chip-based platform should allow direct and real-time analysis or screening of ultralow volume of sample.