CONTROL OF CELL BEHAVIOR DURING VERTEBRATE DEVELOPMENT BY SLUG, A ZINC-FINGER GENE
CONTROL OF CELL BEHAVIOR DURING VERTEBRATE DEVELOPMENT BY SLUG, A ZINC-FINGER GENE
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DOI：
10.1126/science.7513443
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发表时间：
1994-05-06
期刊：
影响因子：
56.9
通讯作者：
COOKE, J
中科院分区：
文献类型：
--
作者：
NIETO, MA;SARGENT, MG;COOKE, J
Slug是一种脊椎动物基因，编码蜗牛家族的锌指蛋白，在神经嵴和从原条迁移的中胚层细胞中表达。用鸡鼻涕虫的反义寡核苷酸孵育早期鸡胚。这些寡核苷酸特异性地抑制细胞行为的正常变化，该变化发生在基因表达的新兴身体平面中的两个位点。这种变化是从上皮特征到间充质特征的转变，发生在原肠胚形成期间中胚层的形成和神经嵴从神经管移出时。
Slug, a vertebrate gene encoding a zinc finger protein of the Snail family, is expressed in the neural crest and in mesodermal cells emigrating from the primitive streak. Early chick embryos were incubated with antisense oligonucleotides to chick Slug. These oligonucleotides specifically inhibit the normal change in cell behavior that occurs at the two sites in the emerging body plan in which the gene is expressed. This change, which is the transition from epithelial to mesenchymal character, occurs at the formation of mesoderm during gastrulation and on emigration of the neural crest from the neural tube.