The frequencies of micronuclei induced by cisplatin in newborn rat astrocytes are increased by 50-Hz, 7.5-and 10-mT electromagnetic fields
The frequencies of micronuclei induced by cisplatin in newborn rat astrocytes are increased by 50-Hz, 7.5-and 10-mT electromagnetic fields
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DOI：
10.1007/bf02900806
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发表时间：
2005-05-01
影响因子：
4.7
通讯作者：
Shimizu, Hidesuke
中科院分区：
文献类型：
--
作者：
Miyakoshi, Yuichi;Yoshioka, Hayato;Shimizu, Hidesuke
目的：流行病学研究表明，暴露于环境和职业电磁场（EMF）有助于诱发脑肿瘤，白血病和其他肿瘤。本研究的目的是调查的遗传毒性效应暴露于50 Hz的电磁场，并共同暴露于顺铂，诱变剂和致癌物，和50 Hz的电磁场，使用在体内新生大鼠星形胶质细胞微核assay.Methods：三天大的雄性SD大鼠共暴露于50 Hz的电磁场和1.25或2.5 mg/kg的顺铂。将脑细胞分离成单细胞并培养96小时，然后用吖啶橙子和抗胶质细胞酸性蛋白的抗体染色。结果：电磁场单独暴露大鼠星形胶质细胞，其微核率不增加。然而，与2.5 mg/kg顺铂单独暴露（假暴露，0-mT电磁场）72小时相比，2.5 mg/kg顺铂与电磁场（7.5-和10-mT）联合暴露72小时的微核率显著增加（p
Objectives: Epidemiological studies have suggested that exposure to environmental and occupational electromagnetic fields (EMFs) contribute to the induction of brain tumors, leukemia, and other neoplasms. The aim of this study was to investigate the genotoxic effects of exposure to 50-Hz EMFs, and of co-exposure to cisplatin, a mutagen and carcinogen, and 50-Hz EMFs, using an in vivo newborn rat astrocyte micronucleus assay.Methods: Three day-old male Sprague-Dawley rats were co-exposed to 50-Hz EMFs and 1.25 or 2.5 mg/kg of cisplatin. Brain cells were dissociated into single cells and cultured for 96 hours, then stained with acridine orange and an antibody against glial fibrillary acidic protein. The frequency of micronucleated astrocytes was counted with a fluorescent microscope.Results: The frequency of micronuclei was not increased in rat astrocytes exposed to EMFs alone. However, the frequencies of micronuclei in co-exposure to 2.5 mg/kg cisplatin and EMFs (7.5- and 10-mT) were significantly increased, compared with those in exposure to 2.5 mg/kg cisplatin alone (sham-exposure, 0-mT EMFs) for 72 hours (p