Polyoma virus middle‐T‐transformed PECAM‐1 deficient mouse brain endothelial cells proliferate rapidly in culture and form hemangiomas in mice
Polyoma virus middle‐T‐transformed PECAM‐1 deficient mouse brain endothelial cells proliferate rapidly in culture and form hemangiomas in mice
复制标题
DOI：
10.1002/jcp.20114
复制
发表时间：
2005
影响因子：
5.6
通讯作者：
Terri A. Rothermel;B. Engelhardt;N. Sheibani
中科院分区：
文献类型：
--
作者：
Terri A. Rothermel;B. Engelhardt;N. Sheibani
Wild‐type mouse brain endothelial (bEND) cells transformed with the polyoma virus middle‐T proliferate rapidly in culture and form hemangiomas in mice. These cells express high levels of platelet/endothelial cell adhesion molecule‐1 (PECAM‐1), a molecule shown to be important during hemangioma formation. In this study, we have examined the ability of polyoma virus middle‐T‐transformed mouse bEND cells prepared from PECAM‐1−/− mice to proliferate in culture and form hemangiomas in mice. We show that these cells express a number of endothelial cell markers and share a similar morphology with PECAM‐1+/+ bEND cells. PECAM‐1−/− bEND cells exhibit a limited ability to form tubes in Matrigel and rapidly form hemangioma when injected into nude mice, very similar to PECAM‐1+/+ bEND cells. These cells, however, have increased proliferation, slower migration, altered endothelial cell adhesion molecule expression, and are less adherent when compared to PECAM‐1+/+ bEND cells. Therefore, lack of PECAM‐1 expression impacts polyoma middle‐T‐transformed endothelial cell proliferative, adhesive, and migratory properties without impacting their ability to rapidly form hemangiomas in mice or poorly organize to capillary‐like structures in Matrigel. © 2005 Wiley‐Liss, Inc.
