Sulfate transport in cultured tobacco cells.
Sulfate transport in cultured tobacco cells.
复制标题
培养烟草细胞中的硫酸盐运输。
DOI：
10.1104/pp.55.2.303
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发表时间：
1975
期刊：
影响因子：
7.4
通讯作者：
I. Smith
中科院分区：
文献类型：
--
作者：
I. Smith
烟草对硫酸盐的运输Xanthi)细胞在以L-半胱氨酸或硫酸盐为唯一硫源的条件下培养。两种硫源的转运速率在指数生长期较低，指数生长期增加，在指数生长期达到最大。最初转运速率的增加与细胞内硫酸盐的下降有关，但与细胞内氨基酸含量无关，氨基酸含量在内源硫酸盐库耗尽之前保持相对恒定。先前报道的L-半胱氨酸对硫酸盐转运的抑制是由于半胱氨酸降解为硫酸盐而导致细胞内硫酸盐增加所致。推测细胞内硫酸盐池是调节硫酸盐进入烟草细胞的主要因素。
Sulfate transport by tobacco (Nicotiana tabacum L. var. Xanthi) cells cultured on either l-cysteine or sulfate as a sole sulfur source was measured. The transport rate on either sulfur source was low during pre-exponential growth, increased during exponential growth, and was maximal in late exponential cells. The initial increase in transport rate was correlated with a decline in the intracellular sulfate, but was not correlated with the amino acid content of the cells which remained relatively constant before the depletion of the endogenous sulfate pool. The previously reported inhibition of sulfate transport by l-cysteine was shown to be caused by an elevation in intracellular sulfate resulting from the degradation of cysteine to sulfate. It is proposed that the intracellular sulfate pool is the major factor regulating the entry of sulfate into tobacco cells.