An integrated flow cytometry analysis of 286 mature B cell neoplasms identifies CD13 as a useful marker for diagnostic subtyping
An integrated flow cytometry analysis of 286 mature B cell neoplasms identifies CD13 as a useful marker for diagnostic subtyping
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DOI：
10.1111/ijlh.12909
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发表时间：
2018-12-01
影响因子：
3
通讯作者：
Ohgami, Robert S.
中科院分区：
文献类型：
--
作者：
Lau, Hubert;Nagy, Alexandra;Ohgami, Robert S.
引言CD 13是一种髓系相关抗原，可由一部分B细胞淋巴瘤表达;然而，其与其他B细胞相关抗原表达的意义尚未得到充分表征。方法286例成熟B细胞肿瘤在确诊时行流式细胞术分析。对每个病例的CD 13、CD 45、CD 19、CD 20、CD 5、CD 10、CD 38、CD 22、CD 23、FMC 7和κ和λ轻链的表达进行评估，并与临床病理特征相关。CD 13表达与淋巴浆细胞性淋巴瘤（LPL）（16/26）和FMC 7阳性慢性淋巴细胞性白血病/小淋巴细胞性淋巴瘤（CLL/SLL）（11/30）病例特异性相关。48例滤泡性淋巴瘤（FL）中无CD 13表达（0/48）。在所有B细胞肿瘤中，CD 13表达与FMC 7共表达和κ轻链限制正相关，与CD 10共表达和λ轻链限制负相关。未发现CD 13与B细胞肿瘤的总生存期或无病生存期有显著相关性。结论CD 13在LPL和FMC 7阳性的CLL/SLL中的表达高于其他成熟B细胞淋巴瘤亚型，而在FL中则无表达，可作为诊断亚型的一个有用指标。
Introduction CD13 is a myeloid associated antigen, which may be expressed by a subset of B cell lymphomas; however, the significance of its expression along with other B cell associated antigens is not well characterized. Methods Results Two hundred and eighty-six mature B cell neoplasms with flow cytometric analysis performed at the time of diagnosis were identified. Expression of CD13, CD45, CD19, CD20, CD5, CD10, CD38, CD22, CD23, FMC7, and kappa and lambda light chains was assessed for each case and correlated with clinicopathologic features. CD13 expression was associated specifically with cases of lymphoplasmacytic lymphoma (LPL) (16/26)- and FMC7-positive chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) (11/30). No cases of follicular lymphoma (FL) expressed CD13 (0/48). Across all B cell neoplasms, CD13 expression positively correlated with FMC7 co-expression and kappa light chain restriction and negatively correlated with CD10 co-expression and lambda light chain restriction. No significant association of CD13 with overall or disease free survival in B cell neoplasms was seen. Conclusion CD13 expression is present more often in LPL- and FMC7-positive CLL/SLL than other mature B cell lymphoma subtypes and absent in cases of FL and may be a useful feature for diagnostic subtyping.