The Expression and Function of Notch Pathway Genes in the Developing Rat Eye
The Expression and Function of Notch Pathway Genes in the Developing Rat Eye
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DOI：
10.1523/jneurosci.17-04-01425.1997
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发表时间：
1997-02
期刊：
影响因子：
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通讯作者：
Z. Bao;C. Cepko
中科院分区：
文献类型：
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作者：
Z. Bao;C. Cepko
Notch 基因在多种生物体不同组织的发育中发挥着重要作用。由于脊椎动物的眼睛是模式形成和细胞命运决定的绝佳模型系统，这两个过程都可能涉及 Notch，因此我们检查了发育中的大鼠眼睛中 Notch 1 和 Notch 2 及其配体 Delta 和 Jagged 的表达模式。发现Notch 1和Delta在细胞命运决定和分化期间在神经视网膜中表达。发现Notch 2在视杯的非神经元衍生物中表达，包括色素上皮、视柄和睫状体。锯齿状在视囊、睫状体和晶状体内的不同区域表达，其模式随时间而变化。通过使用无复制能力的逆转录病毒在体内引入Notch 1的组成型活性形式，检查了Notch 1在细胞类型规范和分化中的潜在功能。人们发现这种形式的 Notch 1 会导致异常生长并干扰多种视网膜细胞类型的分化。
The Notch gene plays a role in the development of disparate tissues in multiple organisms. Because the vertebrate eye is an excellent model system for both patterning and cell fate determination, two processes that can involve Notch, we examined the expression patterns of Notch 1 andNotch 2, and their ligands Delta andJagged, in the developing rat eye. Notch 1 and Delta were found to be expressed in the neural retina during the period of cell fate determination and differentiation. Notch 2 was found to be expressed in the non-neuronal derivatives of the optic cup, including the pigment epithelium, optic stalk, and ciliary body. Jagged was expressed in distinct regions within the optic vesicle, ciliary body, and lens, with patterns that changed over time. The potential function of Notch 1 in cell-type specification and differentiation was examined by introducing a constitutively active form of Notch 1 in vivo using a replication-incompetent retrovirus. This form of Notch 1 was found to cause abnormal growth and interfere with the differentiation of multiple retinal cell types.