INHIBITION OF GROWTH OF CHLAMYDIA-TRACHOMATIS BY NONOXYNOL-9 INVITRO
INHIBITION OF GROWTH OF CHLAMYDIA-TRACHOMATIS BY NONOXYNOL-9 INVITRO
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DOI：
10.1128/aac.27.5.724
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发表时间：
1985-01-01
影响因子：
4.9
通讯作者：
MCCORMACK, WM
中科院分区：
文献类型：
--
作者：
BENES, S;MCCORMACK, WM
研究了广泛使用的杀精剂壬苯醇醚9、含壬苯醇醚9的Conceptrol凝胶和Conceptrol赋形剂（不含壬苯醇醚-9）抑制C.研究了环己酰亚胺处理的麦考伊细胞中的沙眼衣原体。Conceptrol溶剂使形成的夹杂物数量减少40-59%，但与剂量无关。添加壬苯醇醚9和含有浓度为100 μ g/ml的壬苯醇醚9的Conceptrol凝胶使内含物的数量减少了84- 95%。延长壬苯醇醚9和衣原体微生物之间的接触时间（90-180 min）不会导致额外的抑制作用。使用较高浓度的衣原体微生物并在接种单层前稀释药物-衣原体混合物，检测了较高浓度的壬苯醇醚9。在100-5000 μ g壬苯醇醚9/ml的浓度下，注意到内含物数量的剂量相关性减少19-84%。
The ability of the widely used spermicide nonoxynol 9, Conceptrol gel containing nonoxynol 9 and Conceptrol vehicle (without nonoxynol-9) to inhibit the formation of inclusions of C. trachomatis in cycloheximide-treated McCoy cells was investigated. Conceptrol vehicle produced a non-dose-related 40-59% reduction of the number of inclusions formed. The addition of nonoxynol 9 and Conceptrol gel containing nonoxynol 9 at a concentration of 100 .mu.g/ml reduced the number of inclusions by 84-95%. Prolongation of contact between nonoxynol 9 and the chlamydial organisms from 90-180 min did not result in additional inhibition. Using a higher concentration of chlamydial organisms and diluting the drug-chlamydia mixture before inoculation of the monolayers, higher concentrations of nonoxynol 9 were tested. At concentrations of 100-5000 .mu.g of nonoxynol 9/ml, a dose-related 19-84% reduction of the number of inclusions was noted.