Cytoskeleton Dynamics Are Necessary for Early Events of Lateral Root Initiation in Arabidopsis
Cytoskeleton Dynamics Are Necessary for Early Events of Lateral Root Initiation in Arabidopsis
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DOI：
10.1016/j.cub.2019.06.039
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发表时间：
2019-08-05
期刊：
影响因子：
9.2
通讯作者：
Maizel, Alexis
中科院分区：
文献类型：
--
作者：
Barro, Amaya Vilches;Stoeckle, Dorothee;Maizel, Alexis
植物细胞如何重建分化生长以启动器官尚不清楚。侧根的形态发生依赖于最初对称的建立细胞的不对称分裂。在分裂之前，这些细胞有严格控制的不对称径向扩张。允许和确保这些事件协调的细胞机制尚不清楚。在这里，我们定量分析了微管和f -肌动蛋白在侧根形成过程中的动力学。利用突变体、药理学和组织特异性遗传扰动，我们发现微管和f -肌动蛋白网络的动态重组对于创始细胞的不对称扩张是必要的。这种细胞骨架重塑与中柱鞘和内胚层中的生长素信号交织在一起，以使创始细胞获得启动侧根发育所需的基本极性。我们的研究结果揭示了拟南芥受精卵和侧根建立细胞之间的细胞重塑和极化策略的保守性。我们提出在胚胎和胚胎后器官发生过程中，生长素驱动的细胞骨架的协调重组许可了细胞的不对称生长和分裂。
How plant cells re-establish differential growth to initiate organs is poorly understood. Morphogenesis of lateral roots relies on the asymmetric cell division of initially symmetric founder cells. This division is preceded by the tightly controlled asymmetric radial expansion of these cells. The cellular mechanisms that license and ensure the coordination of these events are unknown. Here, we quantitatively analyze microtubule and F-actin dynamics during lateral root initiation. Using mutants and pharmacological and tissue-specific genetic perturbations, we show that dynamic reorganization of both microtubule and F-actin networks is necessary for the asymmetric expansion of the founder cells. This cytoskeleton remodeling intertwines with auxin signaling in the pericycle and endodermis in order for founder cells to acquire a basic polarity required for initiating lateral root development. Our results reveal the conservation of cell remodeling and polarization strategies between the Arabidopsis zygote and lateral root founder cells. We propose that coordinated, auxin-driven reorganization of the cytoskeleton licenses asymmetric cell growth and divisions during embryonic and post-embryonic organogenesis.