DIMETHYLSULFOXIDE PREVENTS DNA NICKING MEDIATED BY IONIZING-RADIATION OR IRON HYDROGEN-PEROXIDE GENERATED HYDROXYL RADICAL
DIMETHYLSULFOXIDE PREVENTS DNA NICKING MEDIATED BY IONIZING-RADIATION OR IRON HYDROGEN-PEROXIDE GENERATED HYDROXYL RADICAL
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DOI：
10.1073/pnas.78.2.1001
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
PETTIJOHN, DE
中科院分区：
文献类型：
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作者：
REPINE, JE;PFENNINGER, OW;PETTIJOHN, DE
由γ诱导的单链DNA断裂中，辐照时，80%被羟基自由基（OH）清除剂二甲基亚砜（Me 2SO）阻止;在该过程中，作为OH和Me 2SO相互作用的产物产生CH 4。相反，Me 2SO完全阻断了Fe/H2 O2体系的DNA切口，该体系从Me 2SO产生·OH但产生较少量的CH 4。由于Me 2SO阻止了来自更有效的Fe/H2O系统的DNA断裂，但仅阻断了80%的辐射介导的切口，因此结果表明，OH导致了80%的DNA单链断裂，而剩余的20%是由于不涉及OH的相互作用。
Of the single-stranded DNA breaks induced by .gamma.-irradiation, 80% were prevented by the hydroxyl radical (.cntdot.OH) scavenger dimethyl sulfoxide (Me2SO); CH4 was generated in the process as a product of the interaction of .cntdot.OH and Me2SO. In contrast, Me2SO completely blocked DNA nicking by an Fe/H2O2 system which produces .cntdot.OH but smaller amounts of CH4 from Me2SO. Because Me2SO prevented DNA breaks from the more efficient Fe/H2O system but only blocked 80% of irradiation-mediated nicking, the results suggest that .cntdot.OH is responsible for 80% of the DNA single-strand breaks and the remaining 20% is due to interactions not involving .cntdot.OH.