Cryopreserved cell-laden alginate microgel bioink for 3D bioprinting of living tissues
Cryopreserved cell-laden alginate microgel bioink for 3D bioprinting of living tissues
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DOI：
10.1016/j.mtchem.2018.11.009
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发表时间：
2019-06-01
影响因子：
7.3
通讯作者：
Alsberg, E.
中科院分区：
文献类型：
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作者：
Jeon, O.;Lee, Y. B.;Alsberg, E.
满载细胞的微凝胶已被用作组织构建块，以创建三维（3D）组织和器官。然而，传统的组装方法不能用于制造具有生物力学和结构复杂性的功能性组织结构。在这项研究中，我们提出了定向组装细胞负载双交联海藻酸盐微凝胶，包括氧化和甲基丙烯酸海藻酸盐（OMA）。承载细胞的OMA微凝胶可以在低强度紫外线下直接组装成明确的3D形状和结构。含有干细胞的OMA微凝胶可以成功地冷冻保存长期储存和按需应用，并且回收的包被细胞与新鲜处理的干细胞保持相同的活力和功能。最后，我们已经成功地证明，通过悬浮水凝胶的自由形式可逆嵌入（FRESH） 3D生物打印，装载细胞的微凝胶可以组装成复杂的3D组织结构。这种高度创新的自下而上的策略使用FRESH 3D生物打印满载细胞的OMA微凝胶，可低温保存，为定制和仿生3D组织结构的制造提供了强大且高度可扩展的工具。(C) 2018 Elsevier Ltd.版权所有。
Cell-laden microgels have been used as tissue building blocks to create three-dimensional (3D) tissues and organs. However, traditional assembly methods cannot be used to fabricate functional tissue constructs with biomechanical and structural complexity. In this study, we present directed assembly of cell-laden dual-crosslinkable alginate microgels that comprised oxidized and methacrylated alginate (OMA). Cell-laden OMA microgels can be directly assembled into well-defined 3D shapes and structures under low-level ultraviolet light. Stem cell-laden OMA microgels can be successfully cryopreserved for long-term storage and on-demand applications, and the recovered encapsulated cells maintained equivalent viability and functionality to the freshly processed stem cells. Finally, we have successfully demonstrated that cell-laden microgels can be assembled into complicated 3D tissue structures via freeform reversible embedding of suspended hydrogels (FRESH) 3D bioprinting. This highly innovative bottom-up strategy using FRESH 3D bioprinting of cell-laden OMA microgels, which are cryopreservable, provides a powerful and highly scalable tool for fabrication of customized and biomimetic 3D tissue constructs. (C) 2018 Elsevier Ltd. All rights reserved.