Tissue Levels of Stefin A and Stefin B in Hepatocellular Carcinoma
Tissue Levels of Stefin A and Stefin B in Hepatocellular Carcinoma
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DOI：
10.1002/ar.23311
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发表时间：
2016-04-01
影响因子：
2
通讯作者：
Xie, Qun
中科院分区：
文献类型：
--
作者：
Lin, Yang-Yuan;Chen, Zhi-Wei;Xie, Qun
Stefins已被报道与各种恶性肿瘤的进展和转移有关。然而，stefins在肝细胞癌（HCC）中的表达尚未明确。本研究采用免疫组化染色法测定85例原发性肝癌组织、85例周围非癌组织和9例正常肝组织中stein A和stein B蛋白水平，实时聚合酶链反应测定mRNA水平。评估组织蛋白酶B、组织蛋白酶D的免疫组化染色及组织蛋白酶与stefins的比值。肝癌组织中stein A和stein B的mRNA表达量分别显著高于周围非癌组织和正常肝组织。stefin A和stefin B与肝癌的淋巴结转移、肿瘤大小和Edmondson分级呈显著正相关。单因素和多因素分析显示，Edmondson分级和stein B表达是与HCC淋巴结转移风险相关的独立因素。肝细胞癌组组织蛋白酶B与肝细胞癌A、组织蛋白酶D与肝细胞癌A、组织蛋白酶B与肝细胞癌B、组织蛋白酶D与肝细胞癌B比值均显著高于周围非癌组。组织蛋白酶与癌组织蛋白酶的比值（组织蛋白酶B/癌组织蛋白酶A、组织蛋白酶B/癌组织蛋白酶B和组织蛋白酶D/癌组织蛋白酶B）与淋巴结转移呈显著正相关。我们认为stefin A和stefin B的高表达可能是促进HCC发生和转移的重要因素。(C) 2016 Wiley期刊公司
Stefins have been reported to be associated with the progression and metastasis of various malignant tumors. However, the expressions of stefins in hepatocellular carcinoma (HCC) have not been well-defined. In this study, the protein levels of stefin A and stefin B were assessed by immunohistochemical staining, and the mRNA levels were quantified by real-time polymerase chain reaction in 85 primary HCC tissues, 85 surrounding non-cancerous tissues, and 9 normal hepatic tissues. The immunohistochemical staining of cathepsin B and cathepsin D, and the ratio of cathepsins to stefins were assessed. The mRNA expressions of stefin A and stefin B in HCC tissues were significantly higher than surrounding noncancerous tissues and normal hepatic tissues, respectively. A significant positive relationship of stefin A and stefin B was found with node metastasis, tumor size, and Edmondson grade for HCC. Univariate and multivariate analyses revealed that Edmondson grade and stefin B expression were independent factors associated with the risk of lymph node metastasis in HCC. The ratios of cathepsin B to stefin A, cathepsin D to stefin A, cathepsin B to stefin B and cathepsin D to stefin B of the HCC group were significantly higher than that of the surrounding noncancerous group. A significant positive correlation between the ratio of cathepsins to stefins (cathepsin B/stefin A, cathepsin B/stefin B and cathepsin D/stefin B) and node metastasis was demonstrated. We concluded that high expressions of stefin A and stefin B may be an important factor contributing to the development and metastasis of HCC. (C) 2016 Wiley Periodicals, Inc.