Comparison of a new chemiluminescent immunoassay for von Willebrand factor activity with the ristocetin cofactor-induced platelet agglutination method
Comparison of a new chemiluminescent immunoassay for von Willebrand factor activity with the ristocetin cofactor-induced platelet agglutination method
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DOI：
10.1111/hae.12203
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发表时间：
2013-11-01
期刊：
影响因子：
3.9
通讯作者：
Aznar, J. A.
中科院分区：
文献类型：
--
作者：
Cabrera, N.;Moret, A.;Aznar, J. A.
测量血管性血友病因子 (VWF) 活性对于诊断血管性血友病 (VWD) 至关重要。 VWF 活性通常根据瑞斯托菌素辅因子 (VWF:RCo) 的测量进行评估。然而，该测试在技术上具有挑战性，并且具有很高的分析内和分析间变异性。一种新的自动化学发光免疫测定 VWF 活性最近已投入商业使用（HemosIL AcuStar von Willebrand Factor Ristocetin Cofactor Activity）。本研究的主要目的是评估这种新方法并将其与作为参考方法的 VWF:RCo 测定进行比较。我们研究了 91 个样本、18 个健康志愿者样本和 73 个患者样本（VWF:RCo 水平）
Measuring von Willebrand factor (VWF) activity is essential for the diagnosis of von Willebrand disease (VWD). The VWF activity is usually assessed based on measurement of the ristocetin cofactor (VWF:RCo). However, that test is technically challenging and has high intra- and inter-assay variabilities. A new automated chemiluminescent immunoassay VWF activity has recently become commercially available (HemosIL AcuStar von Willebrand Factor Ristocetin Cofactor Activity). The main objective of this study was to evaluate this new method and to compare it with the VWF:RCo assay as the reference method. We studied 91 samples, 18 healthy volunteers samples and 73 samples from patients (VWF:RCo level