Inactivation of RNase P in Escherichia coli significantly changes post-transcriptional RNA metabolism.
Inactivation of RNase P in Escherichia coli significantly changes post-transcriptional RNA metabolism.
复制标题
DOI：
10.1111/mmi.14808
复制
发表时间：
2022-01
影响因子：
3.6
通讯作者：
Kushner SR
中科院分区：
文献类型：
--
作者：
Mohanty BK;Kushner SR
核糖核酸酶P （RNase P）是所有生物中trna的5'端成熟所必需的，已被证明在大肠杆菌RNA代谢的其他方面发挥有限的作用。利用rna测序（RNA-seq），我们证明RNase P失活影响大肠杆菌中约46%的表达转录物的丰度，并提供证据表明其基本功能是其从多顺反子tRNA转录物中产生前tRNA的能力。RNA-seq结果与已发表的数据和75/83个转录本（mrna、sRNAs和tRNAs）的northern blot分析一致。RNase P突变体转录物丰度的变化也与其半衰期的变化相关。使严格反应失活并不会改变rnpA49的表型。最值得注意的是，在半胱氨酸调控基因、多种毒素-抗毒素模块和sigma s控制基因中，所有基因的转录本丰度都有所增加。令人惊讶的是，多聚(A)聚合酶（PAP I）调节了约10%受RNase P影响的转录本的丰度。RNase P、RNase E和RNase III突变体的转录组比较表明，它们影响不同的底物。总之，我们的工作强烈表明，RNase P在大肠杆菌转录后RNA代谢的各个方面都起着重要作用。众所周知，核糖核酸酶RNase P对于所有生命王国中所有trna的5'端成熟至关重要。我们对rnpA49温度敏感突变体的转录组进行的RNA-seq分析显示，该酶还影响trna以外的RNA丰度，表明它在大肠杆菌转录后RNA代谢中起主要作用。在没有RNase P的情况下，PAP I介导的聚腺苷化可调节约10%的转录本的丰度。
Ribonuclease P (RNase P), which is required for the 5'-end maturation of tRNAs in every organism, has been shown to play a limited role in other aspects of RNA metabolism in Escherichia coli. Using RNA-sequencing (RNA-seq), we demonstrate that RNase P inactivation affects the abundances of ~46% of the expressed transcripts in E. coli and provide evidence that its essential function is its ability to generate pre-tRNAs from polycistronic tRNA transcripts. The RNA-seq results agreed with the published data and northern blot analyses of 75/83 transcripts (mRNAs, sRNAs and tRNAs). Changes in transcript abundances in the RNase P mutant also correlated with changes in their half-lives. Inactivating the stringent response did not alter the rnpA49 phenotype. Most notably, increases in the transcript abundances were observed for all genes in the cysteine regulons, multiple toxin-antitoxin modules, and sigma S-controlled genes. Surprisingly, poly(A) polymerase (PAP I) modulated the abundances of ~10% of the transcripts affected by RNase P. A comparison of the transcriptomes of RNase P, RNase E and RNase III mutants suggest that they affect distinct substrates. Together, our work strongly indicates that RNase P is a major player in all aspects of post-transcriptional RNA metabolism in E. coli. The ribonuclease RNase P is known to be essential for the 5'-end maturation of every tRNAs in all kingdom of life. Our RNA-seq analyses of the transcriptome of the rnpA49 temperature sensitive mutant showed that the enzyme also affects the abundance of RNAs other than tRNAs, suggesting it plays a major role in post-transcriptional RNA metabolism in E. coli. PAP I mediated polyadenylation modulates the abundance of ~10% of the transcripts in the absence of RNase P.
登录
查看更多内容
影响因子：
3.2
作者：
Imlay, Karin R. Chonoles;Korshunov, Sergey;Imlay, James A.
通讯作者：
Imlay, James A.
影响因子：
4.8
作者：
Blum, E;Carpousis, AJ;Higgins, CF
通讯作者：
Higgins, CF
影响因子：
14.9
作者：
Clarke JE;Kime L;Romero A D;McDowall KJ
通讯作者：
McDowall KJ
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
64.5
作者：
GUERRIERTAKADA, C;GARDINER, K;ALTMAN, S
通讯作者：
ALTMAN, S