Reprogramming cells to study vacuolar development.
Reprogramming cells to study vacuolar development.
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DOI：
10.3389/fpls.2013.00493
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发表时间：
2013
影响因子：
5.6
通讯作者：
Menassa R
中科院分区：
文献类型：
--
作者：
Feeney M;Frigerio L;Kohalmi SE;Cui Y;Menassa R
在营养和胚胎发育过渡期间，植物细胞进行大规模重组，以支持后续发育阶段将发生的活动。研究在这些短暂的过渡期内发生的细胞和亚细胞变化有时会带来挑战，特别是在处理拟南芥胚胎和种子组织时。作为一种补充方法，细胞重编程可以用作研究另一种更容易获得的组织类型中的这些细胞变化的工具。为了对细胞进行重新编程，可以对在建立或抑制种子发育程序中发挥关键作用的特定调节因子进行基因操作，从而改变细胞命运。在不同的发育阶段，液泡呈现不同的功能和形态以响应细胞不断变化的需求。裂解液泡（LV）和蛋白质储存液泡（PSV）是拟南芥等开花植物中发现的两种主要液泡类型。尽管两者在形态上不同并具有独特的功能，但它们也具有一些相似的活动。由于两种液泡类型在植物细胞中的共存时间很短，因此它们如何相互替换一直是人们长期以来的好奇心。为了研究 LV 到 PSV 的转变，LEAFY COTYLEDON2（种子发育的关键转录调节因子）在营养细胞中过度表达，以激活种子发育程序。在细胞水平上，观察到拟南芥叶 LV 转化为 PSV 样细胞器。这为进一步研究阐明 LV 到 PSV 转变的机制提供了机会。总的来说，这个例子证明了细胞重编程作为研究发育转变期间发生的细胞过程的方法的潜在用途。
During vegetative and embryonic developmental transitions, plant cells are massively reorganized to support the activities that will take place during the subsequent developmental phase. Studying cellular and subcellular changes that occur during these short transitional periods can sometimes present challenges, especially when dealing with Arabidopsis thaliana embryo and seed tissues. As a complementary approach, cellular reprogramming can be used as a tool to study these cellular changes in another, more easily accessible, tissue type. To reprogram cells, genetic manipulation of particular regulatory factors that play critical roles in establishing or repressing the seed developmental program can be used to bring about a change of cell fate. During different developmental phases, vacuoles assume different functions and morphologies to respond to the changing needs of the cell. Lytic vacuoles (LVs) and protein storage vacuoles (PSVs) are the two main vacuole types found in flowering plants such as Arabidopsis. Although both are morphologically distinct and carry out unique functions, they also share some similar activities. As the co-existence of the two vacuole types is short-lived in plant cells, how they replace each other has been a long-standing curiosity. To study the LV to PSV transition, LEAFY COTYLEDON2, a key transcriptional regulator of seed development, was overexpressed in vegetative cells to activate the seed developmental program. At the cellular level, Arabidopsis leaf LVs were observed to convert to PSV-like organelles. This presents the opportunity for further research to elucidate the mechanism of LV to PSV transitions. Overall, this example demonstrates the potential usefulness of cellular reprogramming as a method to study cellular processes that occur during developmental transitions.
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影响因子：
3.9
作者：
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