SURVIVAL OF CULTURED ALLOGENEIC KERATINOCYTES TRANSPLANTED TO DEEP DERMAL BED ASSESSED WITH PROBE SPECIFIC FOR Y-CHROMOSOME
SURVIVAL OF CULTURED ALLOGENEIC KERATINOCYTES TRANSPLANTED TO DEEP DERMAL BED ASSESSED WITH PROBE SPECIFIC FOR Y-CHROMOSOME
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DOI：
10.1136/bmj.298.6678.917
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发表时间：
1989-04-08
影响因子：
105.7
通讯作者：
LEIGH, I
中科院分区：
文献类型：
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作者：
BRAIN, A;PURKIS, P;LEIGH, I
为了确定培养的同种异体角质形成细胞移植到真皮深层床的存活率，19例患者的24个纹身被深度剃须切除，移植了来自异性供体的培养的同种异体角质形成细胞片。携带Y染色体的细胞通过生物素化Y探针（pHY 2.1）的原位DNA杂交在移植部位的活检标本中被鉴定出来，并使用免疫过氧化物酶技术进行可视化。移植后1周、2周和3周对培养的同种异体移植物部位进行活检。接受男性培养角质形成细胞移植的女性患者的活检标本中未发现男性细胞，而接受女性培养角质形成细胞移植的男性患者的活检标本中，男性细胞的百分比在男性皮肤的正常范围内。培养的异体角化细胞的有益作用是由于对伤口愈合的影响，而不是形成成功的移植物。
To determine the survival of cultured allogeneic keratinocytes transplanted to a deep dermal bed 24 tattoos that had been removed by deep shave excision in 19 patients were grafted with sheets of cultured allogeneic keratinocytes from donors of the opposite sex. Cells carrying the Y chromosome were identified in biopsy specimens taken from the graft site by in situ DNA hybridisation with a biotinylated Y probe (pHY 2.1) and visualised with a technique using immunoperoxidase. The cultured allograft sites were biopsied one, two, and three weeks after transplantation. No male cells were identified in any biopsy specimen from female patients who were given transplants of male cultured keratinocytes, and all biopsy specimens from male patients, who received female cultured keratinocytes, showed percentages of male cells within the normal range for male skin. The beneficial effects of cultivated allogeneic keratinocytes result from effects on wound healing other than forming a successful graft that "takes".