A disposable DNA sample preparation microfluidic chip for nucleic acid probe assay
A disposable DNA sample preparation microfluidic chip for nucleic acid probe assay
复制标题
一种用于核酸探针检测的一次性DNA样品制备微流控芯片
DOI：
10.1109/memsys.2002.984222
复制
发表时间：
2002
期刊：
影响因子：
--
通讯作者：
E. Yoon
中科院分区：
文献类型：
--
作者：
Joon;Byoung;Hyukjun Nam;D. Park;K. Yun;Jun‐Bo Yoon;Jichang You;E. Yoon
提出了一种用于核酸探针分析的DNA样品制备微流控芯片。微流控芯片由微过滤器、微混合器和DNA纯化芯片三部分组成。我们已经制造了具有2.2 /spl μ/m直径孔阵列的微筛型过滤器。我们还证明了混合可以成功地实现低雷诺数低于50通过使用制造的微混合器。制备的DNA纯化芯片显示出15 ng/cm/sup 2/的结合容量和100拷贝/200 /spl mu/L的最小可提取输入浓度。所提出的微流控芯片可以应用于低成本，一次性样品制备NA探针测定。
A new DNA sample preparation microfluidic chip for Nucleic Acid (NA) probe assay has been proposed. The proposed microfluidic chip is composed of three parts: microfilter, micromixer and DNA purification chip. We have fabricated a microsieve type filter with an array of 2.2 /spl mu/m diameter holes. We have also demonstrated the mixing can be successfully achieved for low Reynolds number below 50 by using the fabricated micromixer. The fabricated DNA purification chip has shown a binding capacity of 15 ng/cm/sup 2/ and a minimum extractable input concentration of 100 copies/200 /spl mu/L. The proposed microfluidic chip can be applied for low-cost, disposable sample preparation of NA probe assays.