Transgenic Indian mustard (Brassica juncea) plants expressing an Arabidopsis phytochelatin synthase (AtPCS1) exhibit enhanced As and Cd tolerance
Transgenic Indian mustard (Brassica juncea) plants expressing an Arabidopsis phytochelatin synthase (AtPCS1) exhibit enhanced As and Cd tolerance
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DOI：
10.1007/s11103-007-9158-7
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发表时间：
2007-07-01
影响因子：
5.1
通讯作者：
Korban, Schuyler S.
中科院分区：
文献类型：
--
作者：
Gasic, Ksenija;Korban, Schuyler S.
植物螯合肽（Phytochelatins，PC）是植物体内合成的巯基活性肽，在植物和其他生物体内重金属和类金属的解毒过程中发挥重要作用。本研究的总体目标是通过在印度芥菜（芥菜L.）中表达拟南芥AtPCS1基因，编码植物螯合素合酶（PC），开发具有增加的耐受性和积累的重金属和类金属的转基因植物。FLAG标记的AtPCS1 gDNA在其天然启动子下在印度芥菜中表达，并且转基因pcs系已与野生型植物进行了镉（Cd）和砷（As）的耐受性和积累的比较。与野生型相比，转基因植株对Cd和As的耐受性显著提高。镉处理的植物的芽有显着更高的浓度的PC和硫醇比野生型植物。野生型植株地上部Cd积累量显著高于转基因植株，而转基因植株地上部As积累量与野生型植株相近。虽然phytochelatin合酶提高了印度芥菜耐受更高水平的重金属镉和类金属As的能力，但它并没有增加这些金属在印度芥菜植物地上组织中的积累潜力。
Phytochelatins (PCs) are post-translationally synthesized thiol reactive peptides that play important roles in detoxification of heavy metal and metalloids in plants and other living organisms. The overall goal of this study is to develop transgenic plants with increased tolerance for and accumulation of heavy metals and metalloids from soil by expressing an Arabidopsis thaliana AtPCS1 gene, encoding phytochelatin synthase (PCs), in Indian mustard (Brassica juncea L.). A FLAG-tagged AtPCS1 gDNA, under its native promoter, is expressed in Indian mustard, and transgenic pcs lines have been compared with wild-type plants for tolerance to and accumulation of cadmium (Cd) and arsenic (As). Compared to wild type plants, transgenic plants exhibit significantly higher tolerance to Cd and As. Shoots of Cdtreated pcs plants have significantly higher concentrations of PCs and thiols than those of wild-type plants. Shoots of wild-type plants accumulated significantly more Cd than those of transgenic plants, while accumulation of As in transgenic plants was similar to that in wild type plants. Although phytochelatin synthase improves the ability of Indian mustard to tolerate higher levels of the heavy metal Cd and the metalloid As, it does not increase the accumulation potential of these metals in the above ground tissues of Indian mustard plants.