Rotifers Ingest Giardia Cysts
Rotifers Ingest Giardia Cysts
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轮虫摄入贾第鞭毛虫囊肿
作者：
J. Trout;Elizabeth J. Walsh;Ronald Fayer
从实验感染的荷斯坦牛犊的粪便中分离出贾第鞭毛虫包囊，对来自6个属的7种轮虫进行了暴露，这些轮虫分别是环虫、平腹轮虫、浮萍轮虫、A轮虫、B轮虫、Platyi轮虫和臂尾藻。用异硫氰酸荧光素标记的单抗预染贾第鞭毛虫包囊，并与活的轮虫混合在3孔特氟隆涂布的显微镜玻片上。用位相对比、差分干涉对比和荧光显微镜观察了生物体。5种轮虫--臂尾轮虫、小斑尾轮虫、A和B轮虫和四角扁尾轮虫摄取了不同数量的包囊，这些包囊在整个观察期内滞留在轮虫体内。轮虫摄取贾第虫包囊可能是减少水污染的一种方法。
Seven species of rotifers representing 6 genera, Epiphanes, Plationus, Asplanchna, Philodina species A, Philodina species B, Platyias, and Brachionus, were exposed to Giardia cysts isolated from the feces of experimentally infected holstein calves. Giardia cysts were prestained with a fluorescein isothiocyanate–conjugated monoclonal antibody and mixed with viable rotifers on 3-well Teflon-coated microscope slides. Organisms were observed with phase-contrast, differential interference contrast, and fluorescence microscopy. Five rotifer species, Epiphanes brachionus, Plationus patulus, Philodina (both A and B), and Platyias quadricornis, ingested varying numbers of cysts, which were retained within the rotifers' bodies throughout the observation period. Rotifer ingestion of Giardia cysts may represent a means of reducing water contamination.