A topical heparinoid-containing product improves epidermal permeability barrier homeostasis in mice
A topical heparinoid-containing product improves epidermal permeability barrier homeostasis in mice
复制标题
含有类肝素的外用产品可改善小鼠表皮通透性屏障稳态
DOI：
10.1111/exd.13985
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发表时间：
2019-08-01
影响因子：
3.6
通讯作者：
Man, Mao-Qiang
中科院分区：
文献类型：
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作者：
Yao, Ye;Guo, Pan;Man, Mao-Qiang
由于表皮功能的重要性，包括角质层水合作用和维持渗透性屏障稳态，在各种皮肤和全身性疾病的发病机制中，已经开发了广泛的产品来改善表皮功能。然而，对某些产品（包括含类肝素产品）的潜在机制知之甚少。在本研究中，我们评估了含有类肝素的产品喜疗妥乳膏对表皮渗透屏障功能和与小鼠皮肤渗透屏障形成/维持相关的一组表皮mRNA表达水平的影响。我们的研究结果表明，虽然经皮含水率的基线水平保持不变，但喜疗妥乳膏每天两次治疗7天，显著加速了渗透屏障的恢复，并增加了角质层的水合作用。同时，某些分化标志物相关蛋白、脂质合成酶、角质形成细胞增殖和抗菌肽的表皮mRNA表达水平也显著增加。总之，这些结果提供了外用喜疗妥乳膏改善表皮通透性屏障和抗菌功能的潜在机制。由于其对表皮功能的益处，含类肝素的产品在以异常渗透屏障和抗微生物功能为特征的皮肤状况的管理中可能更有用。
Because of the importance of epidermal functions, including stratum corneum hydration and maintenance of permeability barrier homeostasis, in the pathogenesis of a variety of cutaneous and systemic disorders, a wide range of products has been developed to improve epidermal functions. However, the underlying mechanisms whereby certain products, including heparinoid-containing product, are far little understood. In the present study, we assessed the impact of a heparinoid-containing product, Hirudoid (R) cream, on epidermal permeability barrier function and expression levels of a panel of epidermal mRNA related to the formation/maintenance of the permeability barrier in mouse skin. Our results showed that while the baseline levels of transepidermal water rates remained unchanged, treatment with Hirudoid (R) cream twice daily for 7 days significantly accelerated permeability barrier recovery and increased stratum corneum hydration. In parallel, expression levels of epidermal mRNA for certain differentiation marker-related proteins, lipid synthetic enzymes, keratinocyte proliferation and antimicrobial peptides also increased significantly. Together, these results provide the underlying mechanisms by which topical Hirudoid (R) cream improves epidermal permeability barrier and antimicrobial function. Because of its benefits for epidermal functions, heparinoid-containing product could be more useful in the management of skin conditions, characterized by abnormal permeability barrier and antimicrobial function.