Potassium and sodium measurements at clinical concentrations using phase-modulation fluorometry.
Potassium and sodium measurements at clinical concentrations using phase-modulation fluorometry.
复制标题
使用相位调制荧光测定法测量临床浓度的钾和钠。
DOI：
10.1016/s0925-4005(99)00235-x
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Lakowicz,JosephR
中科院分区：
文献类型：
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作者：
Szmacinski,Henryk;Lakowicz,JosephR
我们描述了使用基于寿命的传感和相位调制荧光法测量全血中存在的浓度的钠和钾的可能性。探针SBFO显示在接近100 mM的钠浓度下显示相位或调制的变化，并且大部分独立于由于钾的干扰效应。由于钠引起的类似光谱变化，发现探针CD 222不适合使用强度比测量法测量血液中的钾。然而，钠在血液浓度接近100 mM时仅导致CD 222寿命的微小变化。因此，CD222和基于寿命的传感可用于在130 mM钠存在下测量钾的血液水平。类似地，钠仅引起钾探针PBFI的寿命的适度变化，其显示钾依赖性寿命。对于CD222和PBFI，血钠水平的存在使表观钾解离常数增加到血液生理范围内。总之，这些结果证明了在血液或血清中存在的细胞外阳离子浓度下基于寿命感测钠和钾的可能性。
We describe the possibility of measuring sodium and potassium at the concentrations present in whole blood using lifetime-based sensing and phase-modulation fluorometry. The probe SBFO was shown to display changes in phase or modulation at sodium concentrations near 100 mM, and to be mostly independent of interfering effects due to potassium. The probe CD222 was found unsuitable for measurements of potassium in blood using intensity-ratio measurements, due to similar spectral changes induced by sodium. However, sodium at blood concentrations near 100 mM causes only a minor change in the lifetime of CD222. Hence, CD222 and lifetime-based sensing can be used to measure blood levels of potassium in the presence of 130 mM sodium. Similarly, sodium causes only a modest change in the lifetime of the potassium probe PBFI, which displays potassium-dependent lifetimes. For both CD222 and PBFI, the presence of blood levels of sodium increases the apparent potassium dissociation constants into the blood physiological range. In total, these results demonstrated the possibility of lifetime-based sensing of sodium and potassium at the extracellular cation concentrations present in blood or blood serum.