Differential expression of Borrelia burgdorferi genes during erythema migrans and Lyme arthritis
Differential expression of Borrelia burgdorferi genes during erythema migrans and Lyme arthritis
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DOI：
10.1086/515684
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发表时间：
1998-10-01
影响因子：
6.4
通讯作者：
Flavell, RA
中科院分区：
文献类型：
--
作者：
Fikrig, E;Feng, W;Flavell, RA
莱姆病的病原体伯氏疏螺旋体在节肢动物载体和哺乳动物宿主中选择性表达基因。用RNA聚合酶链反应检测了3例莱姆病患者的组织标本中特异性B，burgdorferi基因在人类感染过程中的表达。研究ospA是因为OspA被B下调。在蜱虫充血期间，它是一种候选疫苗，正在进行III期临床试验。还评估了p35和p37，因为这些基因在鼠莱姆病疏螺旋体病期间由螺旋体诱导并且在保护性免疫中起作用。p35和p37 mRNA仅在2例游走性红斑和1例莱姆关节炎患者滑膜中检出。在这些组织中均未鉴定到ospA mRNA。这些数据表明，ospA被抑制，而p35和p37在人类感染诱导，这些结果是第一次直接证明的差异B，莱姆病期间的burgdorferi基因表达。
Borrelia burgdorferi the agent of Lyme disease, selectively expresses genes in the arthropod vector and mammalian host. Specific B, burgdorferi gene expression during human infection was examined in tissue specimens, using RNA-polymerase chain reaction, from 3 patients with Lyme disease. ospA was investigated because OspA is down-regulated by B. burgdorferi in ticks during engorgement and is a vaccine candidate in phase III clinical trials. p35 and p37 were also assessed because these genes are induced by spirochetes during murine Lyme borreliosis and play roles in protective immunity. p35 and p37 mRNA mere detected in erythema migrans biopsy specimens from 2 patients and in the synovium of 1 patient with Lyme arthritis. ospA mRNA was not identified in any of these tissues. These data show that ospA is repressed while p35 and p37 are induced in human infection; these results are the first direct demonstration of differential B, burgdorferi gene expression during Lyme disease.