A computer image processing system for quantification of zebrafish behavior
A computer image processing system for quantification of zebrafish behavior
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DOI：
10.1016/j.jneumeth.2003.09.028
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发表时间：
2004-03-15
影响因子：
3
通讯作者：
Sugitani, K
中科院分区：
文献类型：
--
作者：
Kato, S;Nakagawa, T;Sugitani, K
热带斑马鱼（Danio rerio）经常被用于研究发育生物学。在这里，我们开发了一个计算机图像处理系统量化斑马鱼的行为。我们可以通过图形I/O板使用CCD相机获取斑马鱼在水族馆中自由运动的图像。为了获得真实的运动斑马鱼图像，我们需要高的空间分辨率（256 × 256像素）和时间分辨率（10帧/秒）。如此高速的数据分析是使用跳跃搜索方法完成的。通过使用一个小型水族箱，可以追踪新生斑马鱼的踪迹。成年斑马鱼的运动速度（7.2cm/s）远快于新生斑马鱼（1.8cm/s）。此外，通过分离两条鱼的遮挡图像，我们可以获得两条斑马鱼的图像。他们的行为就像一群鱼在追逐另一个。追逐是由两条鱼的距离、角度和接近来定义的。对斑马鱼的追逐率为37%，而对不同的鱼显着降低到不到20%。目前的图像处理系统是一个非常有用的工具，定量评分的集群行为的多条鱼。(C)2003 Elsevier B. V.保留所有权利。
The tropical zebrafish (Danio rerio) has frequently been used for investigating developmental biology. Here, we developed a computer image processing system for quantifying zebrafish behavior. We could acquire an image of zebrafish freely moving in an aquarium using a CCD camera through a graphic I/O board. To acquire the image of moving zebrafish in real time, we required high spatial (256 x 256 pixels) and temporal (10frames/s) resolution. Such a high speed of data analysis was accomplished using a skipping search method. By using a small aquarium, trackings of newborn zebrafish could be traced. The velocity of adult zebrafish (7.2 cm/s) was far faster than that of newbom zebrafish (1.8 cm/s). Furthermore, by separation of occluded images of two fish, we could acquire images of the two zebrafish. They behaved as in a school in which one fish chased the other. The chasing was defined by the distance, angle and approach of the two fishes. The chasing ratio of pairs of zebrafish was 37%, whereas those of pairs of different fish were significantly reduced to less than 20%. The present image processing system is a very useful tool for quantitatively scoring the schooling behavior of multiple fish. (C) 2003 Elsevier B.V. All rights reserved.