Effect of low intensity pulsed ultrasound on healing of an ulna defect filled with a bone graft substitute
Effect of low intensity pulsed ultrasound on healing of an ulna defect filled with a bone graft substitute
复制标题
DOI：
10.1002/jbm.b.30989
复制
发表时间：
2008-07-01
影响因子：
3.4
通讯作者：
Pounder, Neill
中科院分区：
文献类型：
--
作者：
Walsh, William R.;Langdown, Andrew J.;Pounder, Neill
在18只新西兰白色成年兔上建立单侧尺骨缺损1.5cm模型。用β-磷酸三钙骨移植替代物（JAX TCP）填充缺损。一半动物的手术部位每天接受20分钟低强度脉冲超声（LIPUS）治疗。在手术后4周（每组n = 3）或12周（每组n = 6）处死动物，用于放射学和组织学终点。X线摄影显示，在对照组和LIPUS治疗组中，12周时JAX TCP发生了一些吸收。LIPUS治疗没有加速这种吸收。在对照组中，在缺损边缘观察到一些新骨形成，而在4周和12周时，在缺损中心观察到很少的骨形成。相比之下，X线片显示LIPUS处理的动物在整个切片中在4周和12周时有更多的新骨。骨密度（DEXA）显示，在4周时与LIPUS有统计学显著差异，而在12周时没有发现差异。LIPUS处理切片的组织学显示，在缺损处的JAX TCP骨移植替代物颗粒上和颗粒之间形成了新的编织骨。与对照组相比，LIPUS治疗4周时VEGF表达增加，12周时仍保持升高。在两个时间点，CBFA-1表达水平随着LIPUS治疗而升高。LIPUS治疗增加了β-磷酸三钙骨移植替代物愈合尺骨缺损的骨形成。(C)2007 Wiley Periodicals，Inc.
A 1.5 cm unilateral rabbit ulna defect model was performed in 18 adult NZ white rabbits. The defects were filled with a beta-tricalcium phosphate bone graft substitute (JAX TCP). The surgical site in half the animals was treated daily with 20 min of low intensity pulsed ultrasound (LIPUS). Animals were sacrificed at 4 weeks (n = 3 per group) or 12 weeks (n = 6 per group) following surgery for radiographic and histologic endpoints. Radiography revealed some resorption of the JAX TCP by 12 weeks in the control and LIPUS treated groups. LIPUS treatment did not accelerate this resorption. Some new bone formation was noted in the control groups at the defect margins while little bone formed in the center of the defect at 4 and 12 weeks. In contrast, radiographs revealed more new bone at 4 and 12 weeks in the LIPUS treated animals throughout the section. Bone mineral density (DEXA) revealed a statistically significant difference at 4 weeks with LIPUS while no differences were found at 12 weeks. Histology of the LIPUS treated sections demonstrated new woven bone formation on and between the JAX TCP bone graft substitute particles across the defect. VEGF expression was increased with LIPUS treatment at 4 weeks and remained elevated at 12 weeks compared with controls. CBFA-1 expression levels were elevated with LIPUS treatment at both time points. LIPUS treatment increased bone formation in ulna defect healing with a beta-tricalcium phosphate bone graft substitute. (C) 2007 Wiley Periodicals, Inc.