ITS polymorphism within a single strain ofSclerotium rolfsii
ITS polymorphism within a single strain ofSclerotium rolfsii
复制标题
菌核单株ITS多态性
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
N. Matsumoto
中科院分区：
文献类型：
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作者：
I. Okabe;M. Arakawa;N. Matsumoto
从亲本菌核菌S-63的原生质体和菌丝尖端分离到形态不同的菌株63-76和63H1。菌株63-76和63H1菌丝生长减少，菌丝上缺乏钳接。两株菌株在不同引物的RAPD图谱上也存在差异，表明63-76和63H1是从异核体S-63分离出来的同核体。亲本S-63属于ITS-RFLP组1,63-76和63H1的rDNA内部转录间隔区（ITS）的RFLP模式分别与ITS-RFLP组5和组3相似。63-76与5组菌株ITS区域序列相似性大于99%，63H1与3组菌株ITS区域序列相似性大于100%，63-76与63H1 ITS区域序列相似性大于96.3%。亲本菌株S-63直接测序失败。S-63被认为包含ITS类型的第5组和第3组。
Two morphologically distinct strains, 63–76 and 63H1, were isolated from a protoplast and a hyphal tip of the parentalSclerotium rolfsii strain S-63, respectively. Strains 63–76 and 63H1 showed reduced mycelial growth and lacked clamp connections on hyphae. The two strains also differed from each other and from their parent in RAPD patterns generated by several primers, suggesting that 63–76 and 63H1 were homokaryons isolated from the hetereokaryon S-63. Whereas the parent S-63 belonged to ITS-RFLP group 1, RFLP patterns of internal transcribed spacer (ITS) regions of rDNA of 63–76 and 63H1 were similar to those of ITS-RFLP groups 5 and 3, respectively. The sequence similarity of ITS regions were more than 99% between 63–76 and group 5 strains, 100% between 63H1 and the group 3 strain, and 96.3% between 63–76 and 63H1. Direct sequencing failed in the parental strain S-63. S-63 was considered to contain ITS types of groups 5 and 3.