Evaluation of three growth factors in combinations of two for temporomandibular joint disc tissue engineering
Evaluation of three growth factors in combinations of two for temporomandibular joint disc tissue engineering
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DOI：
10.1016/j.archoralbio.2005.07.002
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发表时间：
2006-03-01
影响因子：
3
通讯作者：
Athanasiou, KA
中科院分区：
文献类型：
--
作者：
Almarza, AJ;Athanasiou, KA
组织工程颞下颌关节盘在需要椎间盘切除的治疗中可能有很大的价值。在使用三种生长因子中已经发现了潜在的益处：胰岛素样生长因子-I、碱性成纤维细胞生长因子和转化生长因子-β 1在维持培养物中的椎间盘样组织中。在本研究中，将这三种生长因子成对组合，并在6周内以两种不同浓度进行测试。生长因子的所有组合似乎都是有益的，因为与暴露于生长因子的大多数构建体相比，只有四分之三的对照构建体（不含生长因子）保留了机械完整性。重要的是，所呈现的生长因子的浓度在第6周时对构建体的细胞构成具有显著影响。当使用高浓度的两种生长因子时，与对照相比，构建体中保留的细胞至少是对照的两倍。(c)2005爱思唯尔有限公司保留所有权利。
Tissue engineering of the temporomandibular joint disc could be a great value in treatments that require discectomy. Potential benefit has been found in the use of three growth factors: insulin-like growth factor-I, basic fibroblast growth factor and transforming growth factor-beta 1 in maintaining disc-like tissue in culture. In the present study, these three growth factors were combined in pairs and tested at two different concentrations over a 6-week period. All combinations of the growth factors appear to be beneficial since only three-quarters of the control constructs (without growth factors) retained mechanical integrity, compared with the majority of constructs exposed to growth factors. Importantly, the concentrations of the presented growth factors had a significant impact on the cellularity of constructs at Week 6. When a high concentration of the two growth factors was used, at least twice as many cells remained in the constructs compared with controls. (c) 2005 Elsevier Ltd. All rights reserved.