Coronaviral hypothetical and structural proteins were found in the intestinal surface enterocytes and pneumocytes of severe acute respiratory syndrome (SARS).
Coronaviral hypothetical and structural proteins were found in the intestinal surface enterocytes and pneumocytes of severe acute respiratory syndrome (SARS).
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DOI：
10.1038/modpathol.3800439
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发表时间：
2005-11
期刊：
影响因子：
--
通讯作者：
Lo AW
中科院分区：
文献类型：
--
作者：
Chan WS;Wu C;Chow SC;Cheung T;To KF;Leung WK;Chan PK;Lee KC;Ng HK;Au DM;Lo AW
严重急性呼吸综合征（severeacuterespiratorysyndrome，SARS）是2002年11月至2003年7月在全球范围内肆虐的一种新型传染病。人们对引起SARS的新型冠状病毒的生物学和病理生理学知之甚少。研究了SARS冠状病毒假想蛋白和结构蛋白的组织和细胞分布。从预测的每种蛋白的抗原表位产生针对冠状病毒的假设蛋白（SARS 3a、3b、6、7a和9 b）和结构蛋白（包膜、膜、核衣壳和刺突）的抗体。这些蛋白的存在首先在冠状病毒感染的Vero E6组织培养模型中得到验证。对不同人体组织的免疫组织化学研究，包括一组9例SARS患者的尸检、2例肝活检和肠活检，进一步证实了SARS患者肺细胞和小肠表面肠细胞的细胞质中存在冠状病毒假设蛋白和结构蛋白。使用这种大量的抗体，在其他细胞类型中没有观察到信号，包括逆转录酶-聚合酶链反应报告为阳性的那些器官。SARS患者冠状病毒感染细胞中存在结构蛋白和功能未确定的假设蛋白，且在不同器官中表达模式不同。这些抗肽抗体可用于在组织水平上诊断SARS。
Severe acute respiratory syndrome (SARS) is a newly emerging infectious disease that haunted the world from November 2002 to July 2003. Little is known about the biology and pathophysiology of the novel coronavirus that causes SARS. The tissue and cellular distributions of coronaviral hypothetical and structural proteins in SARS were investigated. Antibodies against the hypothetical (SARS 3a, 3b, 6, 7a and 9b) and structural proteins (envelope, membrane, nucleocapsid and spike) of the coronavirus were generated from predicted antigenic epitopes of each protein. The presence of these proteins were first verified in coronavirus-infected Vero E6 tissue culture model. Immunohistochemical studies on different human tissues, including a cohort of nine autopsies, two liver biopsies and intestinal biopsies of SARS patients, further confirmed the existence of coronaviral hypothetical and structural proteins in the cytoplasm of pneumocytes and small intestinal surface enterocytes in SARS patients. With this vast array of antibodies, no signal was observed in other cell types including those organs in which reverse transcriptase-polymerase chain reactions were reported to be positive. Structural proteins and the functionally undefined hypothetical proteins were expressed in coronavirus-infected cells with distinct expression pattern in different organs in SARS patients. These antipeptide antibodies can be useful for the diagnosis of SARS at the tissue level.
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DOI：
10.1016/j.bbrc.2004.01.076
发表时间：
2004-03-05
影响因子：
3.1
作者：
Wang P;Chen J;Zheng A;Nie Y;Shi X;Wang W;Wang G;Luo M;Liu H;Tan L;Song X;Wang Z;Yin X;Qu X;Wang X;Qing T;Ding M;Deng H
通讯作者：
Deng H
影响因子：
64.8
作者：
Li W;Moore MJ;Vasilieva N;Sui J;Wong SK;Berne MA;Somasundaran M;Sullivan JL;Luzuriaga K;Greenough TC;Choe H;Farzan M
通讯作者：
Farzan M
DOI：
10.1016/s0140-6736(03)13967-0
发表时间：
2003-07-26
期刊：
Lancet (London, England)
影响因子：
--
作者：
Kuiken T;Fouchier RA;Schutten M;Rimmelzwaan GF;van Amerongen G;van Riel D;Laman JD;de Jong T;van Doornum G;Lim W;Ling AE;Chan PK;Tam JS;Zambon MC;Gopal R;Drosten C;van der Werf S;Escriou N;Manuguerra JC;Stöhr K;Peiris JS;Osterhaus AD
通讯作者：
Osterhaus AD
影响因子：
5.4
作者：
Tan, YJ;Teng, E;Hong, WJ
通讯作者：
Hong, WJ
影响因子：
13.5
作者：
Chau, TN;Lee, KC;Lai, CL
通讯作者：
Lai, CL