Aromatase Is Increased in Astrocytes in the Presence of Elevated Pressure
Aromatase Is Increased in Astrocytes in the Presence of Elevated Pressure
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DOI：
10.1210/en.2010-0724
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发表时间：
2011-01-01
期刊：
影响因子：
4.8
通讯作者：
Wigginton, J. G.
中科院分区：
文献类型：
--
作者：
Gatson, J. W.;Simpkins, J. W.;Wigginton, J. G.
创伤性脑损伤（TBI）后，神经元和胶质细胞的进行性损伤和死亡导致脑功能下降。内源性和外源性雌激素可以保护这些脆弱的细胞。在这项研究中，我们假设压力增加导致星形胶质细胞芳香化酶表达和雌激素产生增加。在这项研究中，我们将大鼠胶质瘤（C6）细胞和原代皮质星形胶质细胞增加压力（25毫米汞柱）1，3，6，12，24，48，和72小时。总芳香化酶蛋白和RNA水平分别使用Western分析和RT-PCR测量。此外，我们通过测定雌酮的前体雄烯二酮给药后雌酮的水平来测量芳香酶的活性。我们发现，增加的压力施加到C6细胞和原代皮质星形胶质细胞导致芳香化酶RNA和蛋白质的显着增加。为了扩展这些发现，我们还分析了芳香化酶活性的主要星形胶质细胞在压力增加。我们发现压力的增加导致雄烯二酮转化为雌酮的显著增加（P < 0.01）。总之，我们认为脑外伤后，星形胶质细胞感受到压力的增加，导致芳香化酶的产生和大脑中的活性增加。这些结果可能表明脑雌激素产生机制后，在TBI患者中看到的压力增加。（内分泌学152：207-213，2011）
After traumatic brain injury (TBI), a progressive injury and death of neurons and glia leads to decreased brain function. Endogenous and exogenous estrogens may protect these vulnerable cells. In this study, we hypothesized that increased pressure leads to an increase in aromatase expression and estrogen production in astrocytes. In this study, we subjected rat glioma (C6) cells and primary cortical astrocytes to increased pressure (25 mm Hg) for 1, 3, 6, 12, 24, 48, and 72 h. Total aromatase protein and RNA levels were measured using Western analysis and RT-PCR, respectively. In addition, we measured aromatase activity by assaying estrone levels after administration of its precursor, androstenedione. We found that increased pressure applied to the C6 cells and primary cortical astrocytes resulted in a significant increase in both aromatase RNA and protein. To extend these findings, we also analyzed aromatase activity in the primary astrocytes during increased pressure. We found that increased pressure resulted in a significant (P < 0.01) increase in the conversion of androstenedione to estrone. In conclusion, we propose that after TBI, astrocytes sense increased pressure, leading to an increase in aromatase production and activity in the brain. These results may suggest mechanisms of brain estrogen production after increases in pressure as seen in TBI patients. (Endocrinology 152: 207-213, 2011)