STRUCTURE AND CONTROL OF ASSEMBLY OF CYTOPLASMIC MICROTUBULES IN NORMAL AND TRANSFORMED-CELLS
STRUCTURE AND CONTROL OF ASSEMBLY OF CYTOPLASMIC MICROTUBULES IN NORMAL AND TRANSFORMED-CELLS
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DOI：
10.1002/jss.400050407
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发表时间：
1976-01-01
期刊：
影响因子：
--
通讯作者：
BRINKLEY, BR
中科院分区：
文献类型：
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作者：
FULLER, GM;BRINKLEY, BR
使用针对 6S 微管蛋白 [小牛脑] 的抗体进行的间接免疫荧光分析显示，培养的非转化细胞中存在复杂的细胞质微管复合物 (CMTC)。在被病毒或化学物质自发转化的细胞中，CMTC 发生显着改变。在 Ca 水平升高的情况下，体外和体内微管的组装均受到显着抑制。用低浓度胰蛋白酶短暂处理改变正常细胞的表面，引发细胞质微管的快速分解。提出了一个与微管组装和表面膜调节相关的假设，表明 Ca 是主要的调节信号。细胞系[小鼠、大鼠] BALB/c3T3、SV3T3、RT、RT-2、LN1-2、LZ2-2、AD3-2、BD5-2、LM(TK)-和 RAG 在单层培养物中生长。还使用了源自人皮肤成纤维细胞HSF-CF和HSF以及PA-2的CHO[中国仓鼠卵巢]系和细胞。
Indirect immunofluorescence analyses using antibodies directed against 6S tubulin [calf brain] have shown an elaborate cytoplasmic microtubule complex (CMTC) in nontransformed cells in culture. The CMTC is strikingly altered in cells that have been transformed spontaneously by viruses or by chemicals. Assembly of microtubules in vitro and in vivo is markedly inhibited in the presence of elevated levels of Ca. Alteration of the surface of normal cells by brief treatment with low concentrations of trypsin initiate a rapid breakdown of cytoplasmic microtubules. A hypothesis is presented relating microtubule assembly and surface membrane modulation suggesting that Ca is the primary modulating signal. Cell lines [mouse, rat] BALB/c3T3, SV3T3, RT, RT-2, LN1-2, LZ2-2, AD3-2, BD5-2, LM(TK)- and RAG were grown in monolayer cultures. CHO [Chinese hamster ovary] lines and cells derived from human skin fibroblasts HSF-CF and HSF and PA-2 were also used.