Studies on the isolation and chemical-physical properties of porcine follicle-stimulating hormone.
Studies on the isolation and chemical-physical properties of porcine follicle-stimulating hormone.
复制标题
猪促卵泡激素的分离及理化性质研究。
作者：
R. Whitley;H. Keutmann;R. Ryan
描述了从猪垂体中分离高度纯化的 FSH 的方法。通过卵巢增重生物测定判断纯物质的生物活性为22 NIH-FSH-P2 U/mg。 RIA 几乎没有显示任何免疫反应性 LH，并且凝胶电泳和免疫沉淀仅检测到一种成分。高纯度 FSH 的氨基酸和碳水化合物分析表明存在 1-色氨酸、高含量的天冬氨酸和谷氨酸以及 6% 的唾液酸。未处理和胍处理的猪 FSH 的分子量是通过斯托克斯半径和沉降系数的流体动力学测量来估计的。还针对这种激素开发了高度特异性和敏感性的 RIA。
Methods are described for isolating highly purified FSH from porcine pituitary glands. The biological activity of pure material was 22 NIH-FSH-P2 U/mg, as judged by the ovarian weight augmentation bioassay. Virtually no immunoreactive LH was indicated by RIA, and only one component was detected by both gel electrophoresis and immunoprecipitation. Amino acid and carbohydrate analyses of the highly purified FSH indicated the presence of 1-tryptophan, a high content of aspartic and glutamic acids, and 6% sialic acid. Molecular weights of untreated and guanidine-treated porcine FSH were estimated from hydrodynamic measurements of Stokes radii and sedimentation coefficients. A highly specific and sensitive RIA was also developed for this hormone.