Nucleotide sequence of cDNA containing the complete coding sequence for human lysosomal glucocerebrosidase.
Nucleotide sequence of cDNA containing the complete coding sequence for human lysosomal glucocerebrosidase.
复制标题
含有人溶酶体葡萄糖脑苷脂酶完整编码序列的 cDNA 核苷酸序列。
DOI：
10.1016/s0021-9258(17)42428-8
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Edward I. Ginns
中科院分区：
文献类型：
--
作者：
S. Tsuji;P. Choudary;B. Martin;S. Winfield;J. Barranger;Edward I. Ginns
从人肝癌文库lambda gt11中分离到人葡萄糖脑苷酶的互补DNA克隆。已经确定了1805个碱基对cDNA插入片段的完整核苷酸序列。除了5 ‘和3 ’非翻译区（分别为51和206个碱基对）外，cDNA插入片段还包含1548个完全编码人葡萄糖脑苷酶的碱基对。所有可能的n链糖基化位点被确定。从52号位置的ATG开始的先导多肽的19个氨基酸的检查显示，在肽酶裂解位点有一个疏水核心和一个羧基末端甘氨酸，其特征与其他人类易位蛋白的先导序列一致。从cDNA序列推导出的516个氨基酸计算出的Mr值为57,000，与体外翻译人胎盘mRNA后免疫沉淀鉴定的Mr值一致。
Complementary DNA clones for human glucocerebrosidase were isolated from a human hepatoma library in lambda gt11. The complete nucleotide sequence of the 1805-base pair cDNA insert has been determined. In addition to 5‘ and 3‘ untranslated regions (51 and 206 base pairs, respectively), the cDNA insert contains 1548 base pairs that completely encode human glucocerebrosidase. All possible N-linked glycosylation sites are identified. Examination of the 19 amino acids of the leader polypeptide beginning with the ATG at position 52 revealed a hydrophobic core and a carboxyl-terminal glycine at the peptidase cleavage site, features consistent with the leader sequences described for other human translocated proteins. The Mr of 57,000 calculated from the 516 amino acids deduced from cDNA sequence is in good agreement with that identified by immunoprecipitation following in vitro translation of human placental mRNA.