REGULATION OF LIPOPROTEIN-LIPASE ACTIVITY AND MESSENGER-RNA IN THE MAMMARY-GLAND OF THE LACTATING MOUSE
REGULATION OF LIPOPROTEIN-LIPASE ACTIVITY AND MESSENGER-RNA IN THE MAMMARY-GLAND OF THE LACTATING MOUSE
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DOI：
10.1042/bj2980321
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发表时间：
1994-03-01
影响因子：
4.1
通讯作者：
NEVILLE, MC
中科院分区：
文献类型：
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作者：
JENSEN, DR;GAVIGAN, S;NEVILLE, MC
研究了不同生育阶段和禁食对小鼠乳腺脂蛋白脂酶（LPL）活性和mRNA表达的影响。肝素释放和细胞相关的LPL活性在出生后立即上升，随后1-2天后LPL mRNA增加。在所有生殖阶段，禁食降低乳腺中的LPL活性。在哺乳期，牛奶和肝素释放LPL大幅下降过夜快，而细胞相关的LPL的影响较小，LPL mRNA没有变化。这些研究表明，乳腺LPL活性的细胞外肝素释放部分对生理状态的改变反应最快，通常伴有细胞酶活性的较小变化。LPL mRNA水平的变化，只看到在从怀孕到哺乳的过渡，这些往往遵循，而不是之前，酶活性的变化。我们的结论是，在乳腺脂肪组织中，LPL主要在翻译和翻译后水平进行调节。
We examined the effects of reproductive stage and fasting on lipoprotein lipase (LPL) activity and mRNA in the mouse mammary gland. Heparin-releasable and cell-associated LPL activity rose immediately after birth, followed 1-2 days later by an increase in LPL mRNA. Fasting decreased LPL activity in the mammary gland at all reproductive stages. During lactation, both milk and heparin-releasable LPL were substantially decreased by an overnight fast, whereas cell-associated LPL was less affected and LPL mRNA did not change. These studies indicate that the extracellular, heparin-releasable, fraction of mammary LPL activity responds most rapidly to alterations in physiological state, usually accompanied by smaller changes in cellular enzyme activity. Changes in the level of LPL mRNA were seen only during the transition from pregnancy to lactation, and these tended to follow, rather than precede, changes in enzyme activity. We conclude that in the mammary gland as in adipose tissue, LPL is regulated primarily at the translational and post-translational level.