Molecular regulation of sex change induced by methyltestosterone -feeding and methyltestosterone -feeding withdrawal in the protogynous orange-spotted grouper
Molecular regulation of sex change induced by methyltestosterone -feeding and methyltestosterone -feeding withdrawal in the protogynous orange-spotted grouper
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甲基睾酮摄食和甲基睾酮戒断引起的雌性橙斑石斑鱼性别变化的分子调控
DOI：
10.1093/biolre/iox085
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发表时间：
2017
影响因子：
3.6
通讯作者：
Lin Haoran
中科院分区：
文献类型：
--
作者：
Wang Qing;Liu Yun;Peng Cheng;Wang Xiang;Xiao Ling;Wang Dengdong;Chen Jiaxing;Zhang Haifa;Zhao Huihong;Li Shuisheng;Zhang Yong;Lin Haoran
鱼类的性别认同很容易被外源激素操纵。17-甲基睾酮(MT)治疗已被广泛用于诱导男性命运，但MT治疗引起的性别变化和MT退出的分子和细胞过程尚未得到很好的研究。本研究系统地研究了斜带石斑鱼的性腺组织学、基因表达谱、性类固醇激素水平以及在MT摄食和MT摄食停止诱导的性别变化过程中细胞的变化。在性腺组织学研究的基础上，我们证明了摄食MT引起的性逆转可以分为早期和晚期：早期，雄性生殖细胞和雌性生殖细胞共存，停喂MT导致雌性命运；在晚期，只观察到雄性生殖细胞，停喂MT并不逆转这一过程，导致雄性命运。在早期和晚期，细胞色素P450家族19亚家族A成员1(Cyp19a1a)的基因表达均随着MT的停止而增加。最后，通过示踪双重性别和Mab-3相关转录因子1(Dmrt1)的表达细胞，我们发现生发上皮中的Gonia样细胞可能是性反转过程中发育睾丸的主要生殖细胞来源。总而言之，我们的发现提供了对外源激素诱导性变化潜在的分子和细胞机制的见解。
The sex identity of fish can be easily manipulated by exogenous hormones. Treatment with 17-methyltestosterone (MT) has been widely used to induce a male fate, but the molecular and cellular processes underlying sex changes induced by MT treatments and the withdrawal of MT are not well studied. In this study, we systematically investigated gonadal histology, gene expression profiles, sex steroid hormone levels, and cellular changes during sex changes induced by MT-feeding and MT-feeding withdrawal in the protogynous orange-spotted grouper,Epinephelus coioides. Based on gonadal histology, we demonstrated that MT-feeding-induced sex reversal can be divided into early and late phases: in the early phase, male and female germ cells coexist, and MT-feeding withdrawal leads to a female fate; in the late phase, only male germ cells are observed, and MT-feeding withdrawal does not reverse the process, leading to a male fate. In both the early and late phases,cytochrome P450 family19 subfamily A member 1(cyp19a1a) gene expression increased in response to MT-feeding withdrawal. Finally, by tracingdoublesex- and Mab-3-related transcription factor 1(dmrt1)-expressing cells, we found that gonia-like cells in the germinal epithelium might be the major germ cell sources for developing testes during sex reversal. Collectively, our findings provide insights into the molecular and cellular mechanisms underlying sex changes induced by exogenous hormones.