Biotransformation of 4(20),11-taxadienes by cell suspension cultures of Platycodon grandiflorum
Biotransformation of 4(20),11-taxadienes by cell suspension cultures of Platycodon grandiflorum
复制标题
DOI：
10.1080/1028602031000080397
复制
发表时间：
2003-01-01
影响因子：
1.7
通讯作者：
Guo, DA
中科院分区：
文献类型：
--
作者：
Dai, JG;Guo, HZ;Guo, DA
以桔梗悬浮细胞为载体，对紫杉烷类二萜2 α，5 α，10 β，14 β-四乙酰氧基-4（20），11-紫杉二烯（1）和9 α-羟基-2 α，5 α，10 β，14 β-四乙酰氧基-4（20），11-紫杉二烯（2）进行生物转化。从1和两种产物9 α，10 β-二羟基-2 α，5 α，14 β-三乙酰氧基-4（20），11-紫杉二烯（4）和10 β-羟基-2 α，5 α，9 α，14 β-四乙酰氧基-4（20）得到一种产物10 β-羟基-2 α，5 α，14 β-三乙酰氧基-4（20），11-紫杉二烯（3），从2个化合物中分离得到11-紫杉二烯（5），其中5为新的紫杉烷类化合物。研究了1和2的添加时期对生物转化的影响，结果表明：（1）1的最佳添加时期为菌体生长的对数生长期早期（第6天），此时1的转化率为78%，3的转化率为75%;（2）2的最佳添加时期为菌体生长的对数中期（第12天），此时2的转化率为25.3%，4和5的转化率分别为18.9%和14.5%。
Platycodon grandiflorum cell suspension cultures were employed to biotransform the taxane diterpenoids 2alpha,5alpha, 10beta,14beta-tetraacetoxy-4(20),11-taxadiene ( 1 ) and 9alpha-hydroxy-2alpha, 5alpha,10beta,14beta-tetraacetoxy-4(20),11-taxadiene ( 2 ). One product, 10beta-hydroxy-2alpha,5alpha,14beta-triacetoxy-4(20),11-taxadiene ( 3 ) was obtained from 1 and two products, 9alpha,10beta-dihydroxy-2alpha,5alpha,14beta-triacetoxy-4(20),11-taxadiene ( 4 ) and 10beta-hydroxy-2alpha,5alpha,9alpha,14beta-tetraacetoxy-4(20),11-taxadiene ( 5 ) were obtained from 2 incubated with Platycodon cultured cells respectively, among which 5 is characterized as a new taxoid compound. The effects of the addition stage for 1 and 2 on the biotransformation were investigated and the results revealed that: (1) the optimal addition stage for 1 was in the early logarithmic phase (6th day) of the cell growth period, in which 78% of 1 was converted and the yield for 3 reached 75%; (2) the optimal addition stage for 2 was on the mid-logarithmic phase (12th day) of the cell growth period, in which 25.3% of 2 was converted and the yields for 4 and 5 reached 18.9 and 14.5%, respectively.