DNA barcodes distinguish species of tropical Lepidoptera
DNA barcodes distinguish species of tropical Lepidoptera
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DOI：
10.1073/pnas.0510466103
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发表时间：
2006-01-24
影响因子：
11.1
通讯作者：
Hebert, PDN
中科院分区：
文献类型：
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作者：
Hajibabaei, M;Janzen, DH;Hebert, PDN
虽然对许多生物学研究至关重要，但物种的识别往往很困难。DNA条形码是来自基因组标准化区域的短DNA序列，最近被提议作为促进物种识别和发现的工具。然而，DNA条形码识别标本的物种丰富的热带生物标本的有效性是未知的。在这里，我们表明，细胞色素c氧化酶I DNA条形码有效地区分物种在三个鳞翅目家庭从瓜纳卡斯特保护区在哥斯达黎加西北部。我们发现，97.9%的521种公认的先前的分类工作具有独特的细胞色素c氧化酶I条形码，种间序列重叠的少数情况涉及非常相似的物种。我们还在13个被认为是单一物种的每个物种中发现了两个或更多的条形码簇。这些集群和形态和/或生态性状之间的协变表明被忽视的物种复合体。如果这些结果是普遍的，DNA条形码将大大有助于热带环境中物种的识别和发现。
Although central to much biological research, the identification of species is often difficult. The use of DNA barcodes, short DNA sequences from a standardized region of the genome, has recently been proposed as a tool to facilitate species identification and discovery. However, the effectiveness of DNA barcoding for identifying specimens in species-rich tropical biotas is unknown. Here we show that cytochrome c oxidase I DNA barcodes effectively discriminate among species in three Lepidoptera families from Area de Conservacion Guanacaste in northwestern Costa Rica. We found that 97.9% of the 521 species recognized by prior taxonomic work possess distinctive cytochrome c oxidase I barcodes and that the few instances of interspecific sequence overlap involve very similar species. We also found two or more barcode clusters within each of 13 supposedly single species. Covariation between these clusters and morphological and/or ecological traits indicates overlooked species complexes. if these results are general, DNA barcoding will significantly aid species identification and discovery in tropical settings.