DIFFERENTIAL CYTOKINE EXPRESSION BY HUMAN INTESTINAL EPITHELIAL-CELL LINES - REGULATED EXPRESSION OF INTERLEUKIN-8
DIFFERENTIAL CYTOKINE EXPRESSION BY HUMAN INTESTINAL EPITHELIAL-CELL LINES - REGULATED EXPRESSION OF INTERLEUKIN-8
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DOI：
10.1016/0016-5085(93)91064-o
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发表时间：
1993-12-01
期刊：
影响因子：
29.4
通讯作者：
KAGNOFF, MF
中科院分区：
文献类型：
--
作者：
ECKMANN, L;JUNG, HC;KAGNOFF, MF
背景：为了表征肠上皮细胞在粘膜宿主防御中的作用，我们研究了人结肠上皮细胞组成性细胞因子表达和白细胞介素（IL）-8的调节表达。方法：通过逆转录RNA的聚合酶链反应扩增，评估了人结肠上皮细胞系T84，Caco-2，SW 620和HT 29的细胞因子表达。结果：细胞系组成性表达IL-8和转化生长因子β1的mRNA。此外，一些细胞系表达IL-1α、IL-1β、IL-10和肿瘤坏死因子α（TNFα）的mRNA。没有细胞系表达IL-2、IL-4、IL-5、IL-6或干扰素γ的mRNA。细胞系组成性地或在用生理激动剂TNFα、IL-1β或脂多糖刺激后分泌IL-8。TNFα刺激T84细胞后IL-8分泌增加，同时IL-8 mRNA水平和IL-8基因转录速率增加。IL-8在极化的T84细胞基底外侧表面优先分泌。在进一步的研究中，新鲜分离的人结肠上皮细胞也分泌IL-8。结论：这些结果支持肠上皮细胞与粘膜免疫细胞和炎症细胞之间存在双向通讯的观点。
Background:To characterize the role of intestinal epithelial cells in mucosal host defense, we have examined constitutive cytokine expression and regulated expression of interleukin (IL)-8 by human colonic epithelial cells.Methods:Cytokine expression by the human colonic epithelial cell lines, T84, Caco-2, SW620, and HT29 was assessed by using polymerase chain reaction amplification of reverse-transcribed RNA. Regulated IL-8 expression was analyzed by nuclear run-off assays, Northern blot analysis, and enzymelinked immunosorbent assay.Results:The cell lines constitutively expressed messenger RNA (mRNA) for IL-8 and transforming growth factor β1. In addition, some cell lines expressed mRNA for IL-1α, IL-1β, IL-10 and tumor necrosis factor a (TNFα). None of the cell lines expressed mRNA for IL-2, IL-4, IL-5, IL-6, or interferon gamma. Cell lines secreted IL-8 either constitutively or after stimulation with the physiological agonists TNFα, IL-1β, or lipopolysaccharide. Increased IL-8 secretion after TNFα stimulation of T84cells was accompanied by increased IL-8 mRNA levels and an increased transcription rate of the IL-8 gene. IL-8 was preferentially secreted at the basolateral surface of polarized T84cells. In further studies, freshly isolated human colon epithelial cells also secreted IL-8.Conclusions:These results support the notion of bidirectional communication between intestinal epithelial cells and mucosal immune and inflammatory cells.