Mdodeling a nitrite-dependent anaerobic methane oxidation process: Parameters identification and model evaluation
Mdodeling a nitrite-dependent anaerobic methane oxidation process: Parameters identification and model evaluation
复制标题
模拟依赖亚硝酸盐的厌氧甲烷氧化过程：参数识别和模型评估
DOI：
10.1016/j.biortech.2013.08.001
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发表时间：
2013-11-01
影响因子：
11.4
通讯作者：
Hu, Baolan
中科院分区：
文献类型：
--
作者：
He, Zhanfei;Cai, Chen;Hu, Baolan
亚硝酸盐依赖的厌氧甲烷氧化（n-damo）是最近发现的一个过程，它是由n-damo细菌介导的，该细菌用亚硝酸盐氧化甲烷以产生氮气。本文以Monod型动力学和扩散反应模型为基础，建立了描述该生物过程的动力学模型。通过一系列间歇活性试验和一个连续运行100天的SBR反应器，得到了模型中所需的一些关键动力学参数。生长速率、衰减速率、甲烷亲和常数、亚硝酸盐亲和常数和抑制常数分别为0.0277 ± 0.0022 d（-1）、0.00216 ± 0.00010 d（-1）、0.092 ± 0.005 mmol L-1、在30 ℃下，n-damo细菌分别为0.91 +/- 0.09 mmol L-1和4.1 +/- 0.5 mmol L-1。结果表明，该模型能较好地模拟SBR反应器前76 d的实际运行情况，在常压下，甲烷对SBR反应器的亲和力较高，对SBR反应器的运行没有限制作用，最佳亚硝酸盐浓度为1.92 mmol L-1。(C)2013爱思唯尔有限公司保留所有权利。
Nitrite-dependent anaerobic methane oxidation (n-damo) is a recently discovered process that is intermediated by n-damo bacteria that oxidize methane with nitrite to generate nitrogen gas. In this work, a kinetic model based on Monod type kinetics and diffusion-reaction model was developed to describe the bioprocess. Some key kinetic parameters needed in the model were obtained from a series of batch activity tests and a sequencing batch reactor (SBR) operation over 100 days. The growth rate, decay rate, methane affinity constant, nitrite affinity constant and inhibition constant were 0.0277 +/- 0.0022 d(-1), 0.00216 +/- 0.00010d(-1), 0.092 +/- 0.005 mmol L-1, 0.91 +/- 0.09 mmol L-1 and 4.1 +/- 0.5 mmol L-1 for n-damo bacteria at 30 degrees C, respectively. The results showed that the model could simulate actual performance of the SBR in the first 76 days, that methane was not a limiting factor at atmospheric pressure for its high affinity, and that the optimum nitrite concentration was 1.92 mmol L-1. (C) 2013 Elsevier Ltd. All rights reserved.