Multilineage differentiation and characterization of the human fetal osteoblastic 1.19 cell line: A possible in vitro model of human mesenchymal progenitors
Multilineage differentiation and characterization of the human fetal osteoblastic 1.19 cell line: A possible in vitro model of human mesenchymal progenitors
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DOI：
10.1634/stemcells.2006-0295
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发表时间：
2007-01-01
期刊：
影响因子：
5.2
通讯作者：
Yen, B. Linju
中科院分区：
文献类型：
--
作者：
Yen, Men-Luh;Chien, Chih-Cheng;Yen, B. Linju
人骨髓间充质干细胞(BMMSCs)的体外研究很大程度上依赖于原代培养。尽管这些都是优秀的模型系统，但它们的稀缺性、异构性和有限的使用寿命限制了它们的可用性。这促使研究人员寻找MSCs的其他来源，最近，在包括骨在内的中胚层组织中发现了这样的祖细胞/干细胞群体。因此，我们推测一个经过充分研究并可用于商业用途的克隆人骨祖细胞系--胎儿成骨细胞1.19细胞系(HFOB)--可能具有多系分化潜能。我们发现，未分化的hFOB细胞具有与BMMSCs相似的细胞表面标志，并表达胚胎干细胞相关的多能性基因Oct-4和神经前体标志Nestin。除了成熟的成骨细胞外，hFOB细胞还可以通过多向分化为软骨细胞和脂肪细胞类型的中胚层细胞。此外，与骨髓间充质干细胞一样，在神经诱导条件下，hFOB细胞获得了神经样表型。该人类细胞系一直是正常成骨细胞分化的广泛使用的模型。我们的数据提示，hFOB细胞可能为研究人间充质祖细胞提供一个容易获得、均一、一致的体外模型。
The in vitro study of human bone marrow mesenchymal stromal cells (BMMSCs) has largely depended on the use of primary cultures. Although these are excellent model systems, their scarcity, heterogeneity, and limited lifespan restrict their usefulness. This has led researchers to look for other sources of MSCs, and recently, such a population of progenitor/stem cells has been found in mesodermal tissues, including bone. We therefore hypothesized that a well-studied and commercially available clonal human osteoprogenitor cell line, the fetal osteoblastic 1.19 cell line (hFOB), may have multilineage differentiation potential. We found that undifferentiated hFOB cells possess similar cell surface markers as BMMSCs and also express the embryonic stem cell-related pluripotency gene, Oct-4, as well as the neural progenitor marker nestin. hFOB cells can also undergo multilineage differentiation into the mesodermal lineages of chondrogenic and adipocytic cell types in addition to its predetermined pathway, the mature osteoblast. Moreover, as with BMMSCs, under neural-inducing conditions, hFOB cells acquire a neural-like phenotype. This human cell line has been a widelyused model of normal osteoblast differentiation. Our data suggest that hFOB cells may provide for researchers an easily available, homogeneous, and consistent in vitro model for study of human mesenchymal progenitor cells.