Oxidative Stress-Induced DNA Damage and Apoptosis in Clove Buds-Treated MCF-7 Cells
Oxidative Stress-Induced DNA Damage and Apoptosis in Clove Buds-Treated MCF-7 Cells
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DOI：
10.3390/biom10010139
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发表时间：
2020-01-01
期刊：
影响因子：
5.5
通讯作者：
Mojzis, Jan
中科院分区：
文献类型：
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作者：
Kello, Martin;Takac, Peter;Mojzis, Jan
近几十年来，人们研究了几种香料在预防和治疗癌症方面的潜力。已有文献证明，香料具有抗氧化、抗炎、免疫调节和抗癌作用。其作用机制主要包括诱导肿瘤细胞凋亡、抑制肿瘤的增殖、迁移和侵袭，以及使肿瘤对放化疗增敏。本研究分析了丁香花蕾提取物(CBE)对MCF-7细胞的氧化应激、DNA损伤和应激/存活/凋亡通路的调节作用。我们证明CBE处理诱导caspase依赖的细胞死亡与氧和氮自由基介导的氧化应激增加有关。我们还展示了CBE介导的线粒体促凋亡因子的释放，氧化应激介导的DNA损伤信号的调节，以及Akt，p38MAPK，JNK和Erk1/2通路的调节活性。
In recent decades, several spices have been studied for their potential in the prevention and treatment of cancer. It is documented that spices have antioxidant, anti-inflammatory, immunomodulatory, and anticancer effects. The main mechanisms of spices action included apoptosis induction, proliferation, migration and invasion of tumour inhibition, and sensitization of tumours to radiotherapy and chemotherapy. In this study, the ability of clove buds extract (CBE) to induce oxidative stress, DNA damage, and stress/survival/apoptotic pathways modulation were analysed in MCF-7 cells. We demonstrated that CBE treatment induced intrinsic caspase-dependent cell death associated with increased oxidative stress mediated by oxygen and nitrogen radicals. We showed also the CBE-mediated release of mitochondrial pro-apoptotic factors, signalling of oxidative stress-mediated DNA damage with modulation of cell antioxidant SOD (superoxide dismutase) system, and modulation activity of the Akt, p38 MAPK, JNK and Erk 1/2 pathways.