Serum reference values for leptin in healthy infants.
Serum reference values for leptin in healthy infants.
复制标题
DOI：
10.1371/journal.pone.0113024
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Silvestro L
中科院分区：
文献类型：
--
作者：
Savino F;Rossi L;Benetti S;Petrucci E;Sorrenti M;Silvestro L
关于儿科人群瘦素浓度的报告缺乏婴儿出生后最初几个月的参考值。我们的研究对象是2002年至2012年间的健康足月婴儿，以确定年龄小于18个月的受试者的血清瘦素参考值。采用放射免疫分析法对317例婴儿进行了门诊常规血清瘦素检测。计算中位数和第10 - 90百分位，采用分位数回归获得参考值。将本研究中建立的价值与另一个110名婴儿的独立队列进行比较。婴儿血清瘦素浓度中位数（IQR）为2.37 (3.26)ng/ml （n = 317）。6月龄以下婴儿瘦素浓度中位数为2.81 (3.49)ng/ml (n = 202)， 6 - 12月龄婴儿瘦素浓度中位数为1.44 (2.27)ng/ml (n = 59)， 12-18月龄婴儿瘦素浓度中位数为1.77 (2.05)ng/ml （n = 56）。我们通过估计上、下百分位数得到了基于年龄的瘦素参考值。在整个队列中，男性（n = 168）瘦素浓度中位数（IQR）为2.22 (3.11)ng/ml，女性（n = 149）为2.60 (3.32)ng/ml。根据喂养方式不同，母乳喂养婴儿（n = 188）的血清瘦素中位数浓度高于配方喂养婴儿（n = 129） (2.63 (3.34) ng/ml vs. 2.12 (2.77) ng/ml；p < 0.05)。我们的数据显示，婴儿早期的瘦素浓度没有性别差异。6个月后，瘦素浓度略有下降。我们使用了一个大型队列来证实，母乳喂养的婴儿在出生后的前6个月的血清瘦素水平明显高于配方奶粉喂养的婴儿，尽管这种差异在以后的生活中消失了。在这项研究中，我们根据临床和实验室标准协会（CLSI）定义了健康婴儿出生后18个月的瘦素参考范围。
Reports on leptin concentrations in pediatric populations lack reference values for infants in the first months of life. Our study was conducted on healthy full-term infants between 2002 and 2012 to determine serum leptin reference values in subjects less than 18 months old. Routine outpatient blood tests for serum leptin were performed on 317 infants using a radioimmunoassay method. The median and 10th–90th percentiles were calculated to obtain reference values using quantile regression. Values established in this study were compared with another independent cohort of 110 infants. The median (IQR) serum leptin concentration in the infants was 2.37 (3.26) ng/ml (n = 317). The median leptin concentration was 2.81 (3.49) ng/ml (n = 202) in infants younger than 6 months of age, 1.44 (2.27) ng/ml (n = 59) in infants between 6–12 months of age and 1.77 (2.05) ng/ml (n = 56) in infants between 12–18 months of age. We obtained leptin reference values based on age by estimating the lower and upper percentiles. In the entire cohort, the median (IQR) leptin concentration was 2.22 (3.11) ng/ml in males (n = 168) and 2.60 (3.32) ng/ml in females (n = 149). According to the type of feeding median serum leptin concentration was higher in breast-fed infants (n = 188) than in formula-fed infants (n = 129) (2.63 (3.34) ng/ml vs. 2.12 (2.77) ng/ml; p<0.05). Our data revealed no gender difference in leptin concentration in early infancy. After 6 months of life, leptin concentrations decreased slightly. We used a large cohort to confirm that breast-fed infants had significantly higher serum leptin levels than formula-fed infants during the first 6 months of life, although this difference disappeared later in life. In this study, we defined the leptin reference range in healthy infants in the first 18 months of life according to the Clinical and Laboratory Standards Institute (CLSI).