STREPTOCOCCAL NUCLEASES
STREPTOCOCCAL NUCLEASES
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链球菌核酸酶
DOI：
--
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发表时间：
1967
影响因子：
15.3
通讯作者：
W. Yasmineh
中科院分区：
文献类型：
--
作者：
L. Wannamaker;W. Yasmineh
链球菌dna酶C比A酶或B酶更能抵抗热失活。dna酶A和C对细菌核糖核酸抑制剂没有影响，而B酶则明显受到抑制。长期消化相对大量的DNAse B导致抑制剂的化学和生物破坏。核糖核酸酶和脱氧核糖核酸酶的活性都与B酶有关。这两种活性都需要二价阳离子，并且都受到细菌核糖核酸的抑制。两种活性的比例在不同的制剂和部分热失活后是恒定的。两种活性的相互抑制可以在混合底物体系中得到证明。所提出的证据与B酶是一种既能攻击脱氧核糖核酸又能攻击核糖核酸的单一核酸酶的观点一致。
Streptococcal DNAse C is more resistant to heat inactivation than the A or B enzyme. DNAses A and C are indifferent to the bacterial ribonucleic acid inhibitor whereas the B enzyme is markedly inhibited. Prolonged digestion with relatively large amounts of DNAse B results in chemical and biological destruction of the inhibitor. Ribonuclease as well as deoxyribonuclease activity is associated with the B enzyme. Both activities require divalent cations and both are inhibited by bacterial ribonucleic acid. The ratios of the two activities are constant in various preparations and after partial heat inactivation. Mutual inhibition of the two activities can be demonstrated in mixed substrate systems. The evidence presented is consistent with the view that the B enzyme is a single nuclease which can attack both deoxyribonucleic and ribonucleic acids.