Genome-Wide Identification of Flowering-Time Genes in Brassica Species and Reveals a Correlation between Selective Pressure and Expression Patterns of Vernalization-Pathway Genes in Brassica napus.
Genome-Wide Identification of Flowering-Time Genes in Brassica Species and Reveals a Correlation between Selective Pressure and Expression Patterns of Vernalization-Pathway Genes in Brassica napus.
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甘蓝属植物开花时间基因的全基因组鉴定并揭示甘蓝型油菜春化途径基因的选择压力与表达模式之间的相关性
DOI：
10.3390/ijms19113632
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发表时间：
2018-11-18
影响因子：
5.6
通讯作者：
Lu K
中科院分区：
文献类型：
--
作者：
Li H;Fan Y;Yu J;Chai L;Zhang J;Jiang J;Cui C;Zheng B;Jiang L;Lu K
开花时间是一个重要的农艺性状，直接影响作物的产量和品质。许多开花时间基因已经在模式植物拟南芥中被鉴定和鉴定；然而，这些基因在许多重要的农学意义上的十字花科作物中仍然没有鉴定。在本研究中，我们分别从甘蓝型油菜、甘蓝型油菜和甘蓝中鉴定了1064、510和524个拟南芥开花时间基因的同源基因，发现与衰老和环境温度途径有关的基因比其他开花途径中的基因要少。甘蓝型油菜和甘蓝型油菜开花期基因主要分布在C03染色体上，甘蓝型油菜开花期基因主要分布在A09染色体上。非同义(Ka)/同义替换(Ks)比值的计算表明，春化途径中的开花时间基因经历了比其他途径更大的选择压力。表达分析表明，春化途径基因多数在花器官中表达。大约40%的这些基因在花药中高表达，而开花时间整合基因以高度器官特异性的方式表达。进化选择压力与春化途径基因的广度和表达水平呈负相关。这些发现提供了这三种甘蓝型油菜开花时间基因的完整框架，并为破译甘蓝型油菜基因表达模式与其进化选择压力之间的关系提供了基础。
Flowering time is a key agronomic trait, directly influencing crop yield and quality. Many flowering-time genes have been identified and characterized in the model plant Arabidopsis thaliana; however, these genes remain uncharacterized in many agronomically important Brassica crops. In this study, we identified 1064, 510, and 524 putative orthologs of A. thaliana flowering-time genes from Brassica napus, Brassica rapa, and Brassica oleracea, respectively, and found that genes involved in the aging and ambient temperature pathways were fewer than those in other flowering pathways. Flowering-time genes were distributed mostly on chromosome C03 in B. napus and B. oleracea, and on chromosome A09 in B. rapa. Calculation of non-synonymous (Ka)/synonymous substitution (Ks) ratios suggested that flowering-time genes in vernalization pathways experienced higher selection pressure than those in other pathways. Expression analysis showed that most vernalization-pathway genes were expressed in flowering organs. Approximately 40% of these genes were highly expressed in the anther, whereas flowering-time integrator genes were expressed in a highly organ-specific manner. Evolutionary selection pressures were negatively correlated with the breadth and expression levels of vernalization-pathway genes. These findings provide an integrated framework of flowering-time genes in these three Brassica crops and provide a foundation for deciphering the relationship between gene expression patterns and their evolutionary selection pressures in Brassica napus.
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影响因子：
9.5
作者：
Imaizumi, Takato
通讯作者：
Imaizumi, Takato
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